Detailed information of TA system TA174372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2700148..2700677
• Replicon: chromosome
• Accession: NZ_CP062334
• Organism: Staphylococcus aureus strain NAS_NP_164

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF749_RS13405
• Protein ID: WP_000621175.1
• Coordinates: 2700315..2700677 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF749_RS13400
• Protein ID: WP_000948331.1
• Coordinates: 2700148..2700318 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF749_RS13370 (2695184)	2695184..2695744	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF749_RS13375 (2695953)	2695953..2696432	+	480	WP_001287079.1	hypothetical protein	-
IF749_RS13380 (2696425)	2696425..2698008	+	1584	WP_001294620.1	PH domain-containing protein	-
IF749_RS13385 (2697995)	2697995..2698486	+	492	WP_001205912.1	PH domain-containing protein	-
IF749_RS13390 (2698490)	2698490..2698849	+	360	WP_000581197.1	holo-ACP synthase	-
IF749_RS13395 (2698915)	2698915..2700063	+	1149	WP_001281154.1	alanine racemase	-
IF749_RS13400 (2700148)	2700148..2700318	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF749_RS13405 (2700315)	2700315..2700677	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF749_RS13410 (2701027)	2701027..2702028	+	1002	WP_228567933.1	PP2C family protein-serine/threonine phosphatase	-
IF749_RS13415 (2702147)	2702147..2702473	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF749_RS13420 (2702475)	2702475..2702954	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF749_RS13425 (2702929)	2702929..2703699	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174372 WP_000621175.1 NZ_CP062334:2700315-2700677 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174372 NZ_CP080999:c4146842-4146579 [Mycobacterium malmoense]
GTGACCTATCGCATTGAACTGACCGGAGCCGCCAGGAAGGCGCTGACCGAATCCCTACCCGAGGCAGTGGCCGTCGCGTG
CTGGGAGTTCATTCGAGGCCCGCTCGCCGAGAATCCTCAACGAGTAGGCAAGCCACTCCGCGGCCAGTTGGAGGGGCGGT
ACTCGGCGCGGCGCGGAGAGTTTCGTGTCATCTACCAGATCTTCGACGAACGAGTGGTGGTCCGGATCATCTACATCGCA
CATCGTCGTGACGTTTACCGGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174372 WP_000948331.1 NZ_CP062334:2700148-2700318 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174372 NZ_CP080999:c4147117-4146839 [Mycobacterium malmoense]
ATGTCCACGCTCCCGCTGGCCGAGGTTCGGGCCAACCTGTCCAAGCTGGTTGATGAGGCGGTCCGCACCCATGAGCGAAT
CGAGGTCACTCGGCAAGGTCGTCGGGCAGCCGTGATTCTCAGTGCGGACGACTTCGACTCGATCATGGAAACCCTGGCGA
TCCTCAGCGATCAGGAGCTGATGCGCGAGGTCCGTGCGGCCGAGGCGGAAAGCGGTCAGACCTACACGTTGGACGAGGTG
ACCGCGGAGATGCGCGCCGCCGGCCGGCTACCTCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1