Detailed information of TA system TA174359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 162909..163685
• Replicon: chromosome
• Accession: NZ_CP062334
• Organism: Staphylococcus aureus strain NAS_NP_164

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF749_RS01130
• Protein ID: WP_000031108.1
• Coordinates: 163533..163685 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF749_RS01125
• Protein ID: WP_001251224.1
• Coordinates: 162909..163508 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF749_RS01105 (158825)	158825..160282	+	1458	WP_000649908.1	ABC transporter permease subunit	-
IF749_RS01110 (160275)	160275..160997	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF749_RS01115 (161148)	161148..162275	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF749_RS01120 (162280)	162280..162750	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF749_RS01125 (162909)	162909..163508	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF749_RS01130 (163533)	163533..163685	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF749_RS01135 (164233)	164233..164628	+	396	WP_000901023.1	hypothetical protein	-
IF749_RS01140 (164824)	164824..166209	+	1386	WP_000116229.1	class II fumarate hydratase	-
IF749_RS01145 (166661)	166661..167482	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174359 WP_000031108.1 NZ_CP062334:163533-163685 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174359 NZ_CP080999:c605700-605443 [Mycobacterium malmoense]
GTGAGGCTGGTCTTCACGCCTCTCGGATGGGCCGACTACCAGTACTGGAGCACAACCGACCGAGGGACACAGAAGCGTAT
CAACCGCCTCATCGATGACACCTTGCGTGATCCGTTCGCCGGGATTGGCAAACTTGAGCCGTTACGCCACGCCTTCGCGG
GCGCCTGGTCACGGCGCATCAGCGAGGAACACCGCTTGGTGTACCTCGTCGATGGCGATGACGTCGTGATCGTGCAGGCC
CGGTACCACTACACCTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT174359 WP_001251224.1 NZ_CP062334:162909-163508 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174359 NZ_CP080999:c605942-605697 [Mycobacterium malmoense]
GTGGCGATCACCGCGTCCGAGGCACGCAAGAACTTATTTCCGTTGATCGAGCAGGTCAACAACGACCGCGTCCCGGTGGA
GATCAACTCCCGCCGGGGTGATGCGGTATTGATCTCGCGCGCCGACTACGAAGCCCTGGAAGAGACCGCGCACCTGTTGC
GGTCCCCCGCCAATGCCCGTCGGCTGCTGGAAAGCCTTACCCAGGCGCAGCGTGGCGAACGCGAAGAGCACACGTTGTCC
CGGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4