Detailed information of TA system TA174353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2745024..2745553
• Replicon: chromosome
• Accession: NZ_CP062331
• Organism: Staphylococcus aureus strain NAS_OP_017

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF760_RS13680
• Protein ID: WP_000621175.1
• Coordinates: 2745191..2745553 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF760_RS13675
• Protein ID: WP_000948331.1
• Coordinates: 2745024..2745194 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF760_RS13645 (2740061)	2740061..2740621	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF760_RS13650 (2740829)	2740829..2741308	+	480	WP_001287088.1	hypothetical protein	-
IF760_RS13655 (2741301)	2741301..2742884	+	1584	WP_001294626.1	PH domain-containing protein	-
IF760_RS13660 (2742871)	2742871..2743362	+	492	WP_001205910.1	PH domain-containing protein	-
IF760_RS13665 (2743366)	2743366..2743725	+	360	WP_000581200.1	holo-ACP synthase	-
IF760_RS13670 (2743791)	2743791..2744939	+	1149	WP_240021556.1	alanine racemase	-
IF760_RS13675 (2745024)	2745024..2745194	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF760_RS13680 (2745191)	2745191..2745553	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF760_RS13685 (2745903)	2745903..2746904	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF760_RS13690 (2747023)	2747023..2747349	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF760_RS13695 (2747351)	2747351..2747830	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF760_RS13700 (2747805)	2747805..2748575	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174353 WP_000621175.1 NZ_CP062331:2745191-2745553 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174353 NZ_CP080998:c2482937-2482605 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
GTGAACCGAGGAGAGATCTGGACCGTAGCGGGGGGTGTCTACTCGACAAAACCGCGTCCCGCGGTGATCGTCCAGGATGA
TTTGTTCGATGCCACGATGTCGGTGGCGGTTGCGCCGATCACCAGCGCATTACTGGAGGCTCCGCTAATGCGCATTCGGA
TAGCCGGCGGAGACGGACGGTTATCCGGACTCGACCACGACAGTGACGTGATGATCGACAAGCTCACAACTGTCAGGAGG
TCGAACGTCCACGCGCGAGTCGGTCGGCTGACTGCGGAACAAGTCGTCGAAGTCGAGCGAGCCATGATGGCATTTCTCGG
GCTTGCGCGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174353 WP_000948331.1 NZ_CP062331:2745024-2745194 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174353 NZ_CP080998:c2483140-2482934 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
ATGGCGGTGAGAGACAGGGTCGGCGAGTACCGCCGGCGCATGCGGGAGCGCGGCCTGCGCCCACTGCAAGTCTGGGTGCC
TGACGTGCGCACCGAGAGTTTTGCCGCCGAGGCTCATCGTCAATCGTCACTGGTGGCGCGGGCGGATGTAAACAGCGATG
ATCAGGACTTCATCGAAGCTGTCTCGACGCCATGGGACGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1