Detailed information of TA system TA174352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2725283..2725797
• Replicon: chromosome
• Accession: NZ_CP062331
• Organism: Staphylococcus aureus strain NAS_OP_017

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF760_RS13590
• Protein ID: WP_000581792.1
• Coordinates: 2725588..2725797 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: IF760_RS13585
• Protein ID: WP_000138932.1
• Coordinates: 2725283..2725576 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF760_RS13565 (2720546)	2720546..2721187	+	642	WP_000483153.1	thiamine phosphate synthase	-
IF760_RS13570 (2721273)	2721273..2722145	+	873	WP_000725802.1	membrane protein insertase YidC	-
IF760_RS13575 (2722958)	2722958..2723605	-	648	WP_001187629.1	HD domain-containing protein	-
IF760_RS13580 (2723633)	2723633..2725117	-	1485	WP_000571549.1	cardiolipin synthase	-
IF760_RS13585 (2725283)	2725283..2725576	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF760_RS13590 (2725588)	2725588..2725797	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF760_RS13595 (2725925)	2725925..2726062	+	138	WP_000828354.1	Lmo0850 family protein	-
IF760_RS13600 (2726326)	2726326..2727528	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF760_RS13605 (2727846)	2727846..2728916	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF760_RS13610 (2728931)	2728931..2730289	+	1359	WP_000611462.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T174352 WP_000581792.1 NZ_CP062331:2725588-2725797 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T174352 NZ_CP080998:c2474128-2473535 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
GTGAAGCGGCTCGACCTCGTCGTCGGCCCCAACGGTGCCGGCAAGTCCACCTTCATCGAGCTGACCTTGGCGCCCCTGCT
GCCTGGCAGCGTGGTGGTCAACGCCGACGAGATCGCCAAGCAGCGCTGGCCAGATGACCCCGCCTCACACGCCTATGAAG
CCGCACGGATCGCAGCCGCCACCAGAGAACGCCTGATCGAACTCGGCGAATCCTTCATCGCCGAGACCGTGTTCTCCCAC
CCTTCCAAGCTCGAGCTCCTCTACAGCGCACACCTGCGGGGGTACTCGGTGGTTGTGCACGCGGTTCTCATCCCCGAGCA
GCTCGCCGTGGCCAGGGTGCAACACCGAGTCGCCGCGGGCGGACATTCCGTGCCGGAAGCCAAGATTCGGGAACGCTACC
AACGGCTGTGGGCACTGGTCGCCCTCGCGGCTGCTCGGGCGGACTCAGCGACCTTTTACGACAACAGCGGCATCCGGGGA
CCCCGTATCGTTGCGCAGATGGCCGGCGGCTTCGTCGTGGGCTCCCTCCGCTGGCCGCAGTGGACCCCGGCGGCGCTCAC
CACGTGCTGGCCCAACCCGCCGGATCGCCGCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT174352 WP_000138932.1 NZ_CP062331:2725283-2725576 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT174352 NZ_CP080998:c2474549-2474160 [Mycolicibacterium holsaticum DSM 44478 = JCM 12374]
ATGACTGAGACGGCAGATCGGGTCACGCGGTTTGCCGCGGATCTGCTCGATAGCGCCGCGGCCGAGGGCGCGCGGCAGAG
TCGCTCAGCCAAACAGCAGCTCGATCATTGGGCACGAGTGGGTCGCGCGGTATCAGGACAGCACAGTGCGGCCCGACGCA
AGGTGGAGGCCGCGCTGAGCGGCGCAACTCCGCTGAGCGAGCTGAGCGCCGAGGAAGGTGTGGTGTTCAACGCTGAGATC
TCCGCCGCGATCCAAGAGAGACTGGCCGACACCGACTACGGCGCGACGTTGGCCGGCCGCGGTATCACCACCGTCGCACT
CAACGACGACGGCGAGCTGGTCCAGTACCGCCCCGATGGGACCTCGTCGACGCTGTCAGACCAGCGGTGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13