Detailed information of TA system TA174343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1225946..1226423
• Replicon: chromosome
• Accession: NZ_CP062331
• Organism: Staphylococcus aureus strain NAS_OP_017

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q2G005
• Locus tag: IF760_RS06230
• Protein ID: WP_000052078.1
• Coordinates: 1226235..1226423 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF760_RS06225
• Protein ID: WP_001059690.1
• Coordinates: 1225946..1226206 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF760_RS06190 (1221339)	1221339..1222055	-	717	WP_000150017.1	type I 3-dehydroquinate dehydratase	-
IF760_RS06195 (1222203)	1222203..1222625	+	423	WP_070001992.1	organic hydroperoxide resistance protein	-
IF760_RS06200 (1223024)	1223024..1223518	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF760_RS06205 (1223673)	1223673..1224290	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
IF760_RS06210 (1224363)	1224363..1224944	-	582	WP_001140686.1	histidine phosphatase family protein	-
IF760_RS06215 (1225169)	1225169..1225405	+	237	WP_000368687.1	hypothetical protein	-
IF760_RS06220 (1225402)	1225402..1225605	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF760_RS06225 (1225946)	1225946..1226206	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF760_RS06230 (1226235)	1226235..1226423	+	189	WP_000052078.1	hypothetical protein	Toxin
IF760_RS06235 (1226611)	1226611..1227180	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF760_RS06240 (1227268)	1227268..1227552	+	285	WP_001004395.1	hypothetical protein	-
IF760_RS06245 (1227614)	1227614..1227832	+	219	WP_000005886.1	hypothetical protein	-
IF760_RS06250 (1228328)	1228328..1228528	-	201	WP_001059082.1	cold-shock protein	-
IF760_RS06255 (1228885)	1228885..1229571	-	687	WP_001548082.1	thermonuclease family protein	-
IF760_RS06260 (1229912)	1229912..1230382	-	471	WP_000824472.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selq / selk / vWbp	1200658..1240096	39438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7172.84 Da
• Isoelectric Point: 9.9971

>T174343 WP_000052078.1 NZ_CP062331:1226235-1226423 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174343 NZ_CP080997:101334-101645 [Mycolicibacter heraklionensis]
ATGCGACCCATCCATATCGCCCTGCTCGATAAGTCCAGACCTGTCCTGATCCTCACCCGCGAACTCGTGCGGCCGCATCT
GACCAACGTCACCGTTGCTCCAATTACATCGACCGTCCGAGGCCTGTCCACCGAAGTCCCGGTAGGCGCTGCGAACGGAT
TGAATCAACCGTCCGTCGTCAGTTGCGACAACATCCAAACGATCCCGGTGGTCGACCTTGGTCGGCAGATCGGCTATCTA
CTCGCGTCGCAGGAGTCGGCTCTTGCAGACGCGATCGGTAACGCGTTCGACCTTGATTGGGTCGTGCCATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174343 WP_001059690.1 NZ_CP062331:1225946-1226206 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174343 NZ_CP080997:101098-101334 [Mycolicibacter heraklionensis]
GTGAGCACACAAATTGCCGTCCGCGTGCCCGACGACCTCATCGCTTTCATCGATGACGAAGTTCGCGGCCAACATGCCCG
CAGTCGAGCGGCTGTCGTGCTACGGGCGCTGGAGCGGGAACGTCGCCGTCGCCTCGCTGAACGCGACGCGGTGATCCTCG
CTGCGACCGCGTCAGCGCCGACTGAGATGGACGCGCTGGCCGGCCGATCTGCTCGCACCATCCTCGATATCGACTGA

Structures

Toxin

• AlphaFold DB: A0A0D6HZ40

Antitoxin

• AlphaFold DB: A0A0E0VN56