Detailed information of TA system TA174335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2643831..2644360
• Replicon: chromosome
• Accession: NZ_CP062329
• Organism: Staphylococcus aureus strain NAS_OP_026

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF761_RS12985
• Protein ID: WP_000621175.1
• Coordinates: 2643998..2644360 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF761_RS12980
• Protein ID: WP_000948331.1
• Coordinates: 2643831..2644001 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF761_RS12950 (2638867)	2638867..2639427	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF761_RS12955 (2639636)	2639636..2640115	+	480	WP_001287090.1	hypothetical protein	-
IF761_RS12960 (2640108)	2640108..2641691	+	1584	WP_001294626.1	PH domain-containing protein	-
IF761_RS12965 (2641678)	2641678..2642169	+	492	WP_001205910.1	PH domain-containing protein	-
IF761_RS12970 (2642173)	2642173..2642532	+	360	WP_000581197.1	holo-ACP synthase	-
IF761_RS12975 (2642598)	2642598..2643746	+	1149	WP_001281142.1	alanine racemase	-
IF761_RS12980 (2643831)	2643831..2644001	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF761_RS12985 (2643998)	2643998..2644360	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF761_RS12990 (2644709)	2644709..2645710	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF761_RS12995 (2645829)	2645829..2646155	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF761_RS13000 (2646157)	2646157..2646636	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF761_RS13005 (2646611)	2646611..2647381	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174335 WP_000621175.1 NZ_CP062329:2643998-2644360 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174335 NZ_CP080960:c3535329-3534955 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174335 WP_000948331.1 NZ_CP062329:2643831-2644001 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174335 NZ_CP080960:c3535571-3535329 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1