Detailed information of TA system TA174325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1169288..1169765
• Replicon: chromosome
• Accession: NZ_CP062329
• Organism: Staphylococcus aureus strain NAS_OP_026

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF761_RS05815
• Protein ID: WP_000052076.1
• Coordinates: 1169577..1169765 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF761_RS05810
• Protein ID: WP_001059690.1
• Coordinates: 1169288..1169548 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF761_RS05775 (1164681)	1164681..1165397	-	717	WP_000150012.1	type I 3-dehydroquinate dehydratase	-
IF761_RS05780 (1165545)	1165545..1165967	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
IF761_RS05785 (1166366)	1166366..1166860	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF761_RS05790 (1167015)	1167015..1167632	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
IF761_RS05795 (1167705)	1167705..1168286	-	582	WP_001140693.1	histidine phosphatase family protein	-
IF761_RS05800 (1168511)	1168511..1168747	+	237	WP_000368687.1	hypothetical protein	-
IF761_RS05805 (1168744)	1168744..1168947	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF761_RS05810 (1169288)	1169288..1169548	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF761_RS05815 (1169577)	1169577..1169765	+	189	WP_000052076.1	hypothetical protein	Toxin
IF761_RS05820 (1169953)	1169953..1170522	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
IF761_RS05825 (1170610)	1170610..1170894	+	285	WP_001004395.1	hypothetical protein	-
IF761_RS05830 (1170956)	1170956..1171174	+	219	WP_000005886.1	hypothetical protein	-
IF761_RS05835 (1171670)	1171670..1171870	-	201	WP_001059082.1	cold-shock protein	-
IF761_RS05840 (1172227)	1172227..1172913	-	687	WP_001792695.1	thermonuclease family protein	-
IF761_RS05845 (1173254)	1173254..1173775	-	522	WP_000824484.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174325 WP_000052076.1 NZ_CP062329:1169577-1169765 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174325 NZ_CP080958:2405881-2406159 [Citrobacter amalonaticus]
ATGATCATGAATTTCAGGCACAAGGGATTACGCGACCTGTTTCTTCACGGTAAAACGTCAGGCGTGCTGGCAAAGCACGT
CAAACGTTTGCGACACCGCCTCGCCGTGATTGACGCCGCAGGCTGCGTGAACGATATCAACATGCCCGGTTATCGGCTGC
ATCCTCTGAGCGGCGATCGTGATGGCGTCTGGTCAATATCGGTTTCAGGCAACTGGCGTATGACGTTCGAATTCGTCAAT
GGCGATGCGTATATTTTGGATGACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174325 WP_001059690.1 NZ_CP062329:1169288-1169548 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174325 NZ_CP080958:2406159-2406443 [Citrobacter amalonaticus]
ATGAAAATGGCGAATCATCCCCGCCCAGGGGATATCATTCAGGAAGCGCTGGACGAACTGAACGTCAGTTTGCGTGAATT
TGCCAGAGCAATGGAGATTGCGCCCTCAACCGCCAGCCGGTTACTCACGGGCAAAGCGGCACTCACCCCGGAAATGGCGA
TCAAACTCTCGGTGGTGATCGGCAGCTCCCCGGAGATGTGGCTAAATCTGCAAAATACCTGGAGTCTGGCACAAGCGCAA
AAAAGCGTGGACGTGTCCCGTCTGCGCCGTCTGGCGGTGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56