Detailed information of TA system TA174322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 130672..131448
• Replicon: chromosome
• Accession: NZ_CP062329
• Organism: Staphylococcus aureus strain NAS_OP_026

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF761_RS00830
• Protein ID: WP_000031108.1
• Coordinates: 131296..131448 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF761_RS00825
• Protein ID: WP_001251229.1
• Coordinates: 130672..131271 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF761_RS00805 (126589)	126589..128046	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF761_RS00810 (128039)	128039..128761	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF761_RS00815 (128912)	128912..130039	+	1128	WP_029160281.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF761_RS00820 (130044)	130044..130514	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF761_RS00825 (130672)	130672..131271	+	600	WP_001251229.1	glucosamine-6-phosphate isomerase	Antitoxin
IF761_RS00830 (131296)	131296..131448	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF761_RS00835 (132282)	132282..132677	+	396	WP_000901023.1	hypothetical protein	-
IF761_RS00840 (132873)	132873..134258	+	1386	WP_240029360.1	class II fumarate hydratase	-
IF761_RS00845 (134711)	134711..135532	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174322 WP_000031108.1 NZ_CP062329:131296-131448 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174322 NZ_CP080953:c2427242-2426892 [Hydrogenibacillus sp. N12]
ATGATCGTCCGGCGCGGCGACGTCTTTTTTGCCAATCTTTCCCCCGTGGTCGGCTCCGAGCAGGGGGGCATGCGACCGGT
CCTGGTCATTCAGAACGACATCGGCAACCGCTTTAGCCCGACGGTCATCGTCGCCGCCATCACCTCGCAGATCCAGAAGG
CGAAGCTGCCGACGCACGTCGAGATCTCGGGCAAGGCCCACGGCCTCGATCGGGATTCGGTCATCCTCCTGGAGCAGATT
CGCACGATCGACAAGCAGCGGCTCACGGACAAGATCACCCACCTCGACGACGAGGTGATGAGCCGCGTCAACGAAGCGCT
CATGATCAGCCTCGGCCTGGTCGATTTCTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22329.45 Da
• Isoelectric Point: 5.1445

>AT174322 WP_001251229.1 NZ_CP062329:130672-131271 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174322 NZ_CP080953:c2427526-2427245 [Hydrogenibacillus sp. N12]
TTGGGCAAAGAAGGCGCGGTCAAGCGGATCGTCGTGAGCGTTCCGCAGGACCTTCTGGCCGAGGTGGACGGCCTCGCCGA
GCGGGAGCGGTCCAGCCGGAGCGCCTTCATCCGAGAGGCGATGCGGCACTACGTCTGGGAGAAGCGAAAGGAGCACTTAA
GAGCCCGGATGGAAGCCGGCTATCAGGAGATGGCCCGGATCAACCTGGTCATGGCGGTGGAAGCGCAGCCGGCGGAAGAG
GAAGCCGATCTCACCATTTTGCGCATGGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: X5E2E6