Detailed information of TA system TA174318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2651614..2652143
• Replicon: chromosome
• Accession: NZ_CP062326
• Organism: Staphylococcus aureus strain NAS_OP_043

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF764_RS13000
• Protein ID: WP_000621175.1
• Coordinates: 2651781..2652143 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF764_RS12995
• Protein ID: WP_000948331.1
• Coordinates: 2651614..2651784 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF764_RS12965 (2646651)	2646651..2647211	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
IF764_RS12970 (2647419)	2647419..2647898	+	480	WP_001287077.1	hypothetical protein	-
IF764_RS12975 (2647891)	2647891..2649474	+	1584	WP_001294637.1	PH domain-containing protein	-
IF764_RS12980 (2649461)	2649461..2649952	+	492	WP_001205907.1	PH domain-containing protein	-
IF764_RS12985 (2649956)	2649956..2650315	+	360	WP_000581199.1	holo-ACP synthase	-
IF764_RS12990 (2650381)	2650381..2651529	+	1149	WP_001281140.1	alanine racemase	-
IF764_RS12995 (2651614)	2651614..2651784	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF764_RS13000 (2651781)	2651781..2652143	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF764_RS13005 (2652492)	2652492..2653493	+	1002	WP_042744356.1	PP2C family protein-serine/threonine phosphatase	-
IF764_RS13010 (2653612)	2653612..2653938	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF764_RS13015 (2653940)	2653940..2654419	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
IF764_RS13020 (2654394)	2654394..2655164	+	771	WP_221575077.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174318 WP_000621175.1 NZ_CP062326:2651781-2652143 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174318 NZ_CP080770:2016464-2016925 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTGATAAAGCGTGGTTTATCAGATAGAGAAAAGGCAGGGATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCACGT
AGACACGGCTTTATCTCAGCTGTTCCATTACGCGAAATTGTAGAAGCTTATAATTATGGCGTACGTAACTTGTATGAATT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCGATCACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174318 WP_000948331.1 NZ_CP062326:2651614-2651784 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174318 NZ_CP080770:2016137-2016451 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAACGTTATGAAAGCGGAAATATCAAAAATCTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCGTATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCATCTT
GAAGGAGAATTGACAGATGACGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1