Detailed information of TA system TA174313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1140304..1140781
• Replicon: chromosome
• Accession: NZ_CP062326
• Organism: Staphylococcus aureus strain NAS_OP_043

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF764_RS05675
• Protein ID: WP_000052076.1
• Coordinates: 1140593..1140781 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF764_RS05670
• Protein ID: WP_001059690.1
• Coordinates: 1140304..1140564 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF764_RS05635 (1135697)	1135697..1136413	-	717	WP_000150033.1	type I 3-dehydroquinate dehydratase	-
IF764_RS05640 (1136561)	1136561..1136983	+	423	WP_000974451.1	organic hydroperoxide resistance protein	-
IF764_RS05645 (1137382)	1137382..1137876	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF764_RS05650 (1138032)	1138032..1138649	+	618	WP_000255550.1	LysE/ArgO family amino acid transporter	-
IF764_RS05655 (1138722)	1138722..1139303	-	582	WP_001140696.1	histidine phosphatase family protein	-
IF764_RS05660 (1139527)	1139527..1139763	+	237	WP_000368687.1	hypothetical protein	-
IF764_RS05665 (1139760)	1139760..1139963	-	204	WP_000145160.1	sterile alpha motif-like domain-containing protein	-
IF764_RS05670 (1140304)	1140304..1140564	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF764_RS05675 (1140593)	1140593..1140781	+	189	WP_000052076.1	hypothetical protein	Toxin
IF764_RS05680 (1140969)	1140969..1141538	+	570	WP_001106662.1	lipoprotein N-acylation protein LnsA	-
IF764_RS05685 (1141626)	1141626..1141898	+	273	WP_001004400.1	hypothetical protein	-
IF764_RS05690 (1141971)	1141971..1142189	+	219	WP_000005884.1	hypothetical protein	-
IF764_RS05695 (1142686)	1142686..1142886	-	201	WP_001059082.1	cold-shock protein	-
IF764_RS05700 (1143243)	1143243..1143929	-	687	WP_001622483.1	thermonuclease family protein	-
IF764_RS05705 (1144270)	1144270..1144791	-	522	WP_000824479.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-121)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T174313 WP_000052076.1 NZ_CP062326:1140593-1140781 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T174313 NZ_CP080769:c16980-16579 [Cupriavidus pinatubonensis]
ATGAAGTACCTGCTCGATGCCAACGCGGTCATCGCGATGCTCAAGGGGGAGCGCATCATGCTGGAGCGCCTGCGCGCGCA
CTTGCCAACCGATTTCGGTCTGCCCTCGATCGTGGCGCATGAACTGTACTATGGCGCCTACAAGAGCCAGCGTGCCGTCG
CGAACCTGGCCCGCATTGAAGCGCTACAGTTCGAAGTCGTGCCGTTCGATGCCGAGGACGCCCAACACGCCGGCGAGATT
CGCGCGCAACTGGCGGCGGCCGGCACCCCGATCGGCCCCTACGACGTGCTGATTGCTGGCCAGGCGAGGGCGCGTAACCT
GATTCTGGTGACCCACAACGTACGCGAATTCGCGCGCGTAGCGCAGCTGAACGTCGAAGATTGGCATGCAGGTGGACAGT
GA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT174313 WP_001059690.1 NZ_CP062326:1140304-1140564 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT174313 NZ_CP080769:c17219-16977 [Cupriavidus pinatubonensis]
ATGGAGTTCGCCAAGGTTTTCCGACATGGAGGCTCACAGGCGGTGCGGCTGCCCAAGGACTATCGCTTTGACACTGCCGA
GGTGCGCATCCGTCGTCACGGCGCCGCGGTGATTCTCGAGCCCGTGCCGCAGGACTGGGCCTGGCTGGCGCCGCTGGTGG
GGCCGGTCGATGCCGATTTCGAGTCGGCCGCGGTCGCCCAGCCTGAGGCTCAGGAGCGCCCCGGCCTGGATGTGTTCGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56