Detailed information of TA system TA174312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 120339..121115
• Replicon: chromosome
• Accession: NZ_CP062326
• Organism: Staphylococcus aureus strain NAS_OP_043

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: IF764_RS00770
• Protein ID: WP_000031109.1
• Coordinates: 120963..121115 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF764_RS00765
• Protein ID: WP_001251224.1
• Coordinates: 120339..120938 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF764_RS00745 (116255)	116255..117712	+	1458	WP_000649916.1	ABC transporter permease subunit	-
IF764_RS00750 (117705)	117705..118427	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF764_RS00755 (118578)	118578..119705	+	1128	WP_240029568.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF764_RS00760 (119710)	119710..120180	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF764_RS00765 (120339)	120339..120938	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF764_RS00770 (120963)	120963..121115	+	153	WP_000031109.1	SAS053 family protein	Toxin
IF764_RS00775 (121943)	121943..122338	+	396	WP_000901019.1	hypothetical protein	-
IF764_RS00780 (122534)	122534..123919	+	1386	WP_000116239.1	class II fumarate hydratase	-
IF764_RS00785 (124376)	124376..125197	-	822	WP_000669377.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5950.31 Da
• Isoelectric Point: 3.9075

>T174312 WP_000031109.1 NZ_CP062326:120963-121115 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEESQ

Nucleotide

• Length: 153 bp

>T174312 NZ_CP080767:3885524-3885958 [Cupriavidus pinatubonensis]
ATGATCGTGCTGGACACCAACGTGGTTTCGGAGGCCATGAAGCCGGAGCCGGCGGCAACAGTGCGTGCCTGGCTTGATGA
GCAGGTGGCCGAGACGTTGTACCTGTCCAGCGTCTCGCTGGCCGAATTGCTGTACGGCATTGCTTGCCTGCCGGACGGAC
GTCGCAAGCTTGCGCTGGAAGACGCGTTGGAAGGCCTGATGGCGCTGTTCGCTGGACGTGTGCTGCCCTTCGATCCCGGT
GCAGCACGCTGCTATGCCGAACTCGCGGCGAAGGCTCGCGCGGCAGGCAAAGGTTTTCCCACGCCGGACGGCTACATCGC
CGCCATCGCGACCGCACACGGATTCACGGTTGCCTCACGCGACACGGCTCCGTTCGAAGCTGCCGGCGTTCCGGTGATTG
ATCCCTGGCATTGCGGGCGTGAAGCCCAAAGATAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT174312 WP_001251224.1 NZ_CP062326:120339-120938 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174312 NZ_CP080767:3885270-3885527 [Cupriavidus pinatubonensis]
ATGGCAACGCTAACCATACGCAACCTGCCCGACGAAACCCACCGCGCGTTACGCGTACAGGCGGCCGCGCACGGCCGCAG
CACGGAAGCCGAGGTCCGCGACATTTTGGCGCGTGCCGTGAACCCGCCGGAGCGGGTGAAGATGGGGGAAGCGCTGGCTC
AGCTGGGTCGTCGCCTGGGCCTGACCAACGCCGACGTGGAGGCGCTCGAACAGACGCGTGACAGAACGCCTGCACAACCG
CCGAGGTTCCACGAATGA

Structures

Antitoxin

• AlphaFold DB: W8U4V4