Detailed information of TA system TA174307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2791061..2791590
• Replicon: chromosome
• Accession: NZ_CP062324
• Organism: Staphylococcus aureus strain NAS_OP_063

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF767_RS13960
• Protein ID: WP_000621175.1
• Coordinates: 2791228..2791590 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF767_RS13955
• Protein ID: WP_000948331.1
• Coordinates: 2791061..2791231 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF767_RS13925 (2786098)	2786098..2786658	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF767_RS13930 (2786866)	2786866..2787345	+	480	WP_001287088.1	hypothetical protein	-
IF767_RS13935 (2787338)	2787338..2788921	+	1584	WP_001294626.1	PH domain-containing protein	-
IF767_RS13940 (2788908)	2788908..2789399	+	492	WP_001205910.1	PH domain-containing protein	-
IF767_RS13945 (2789403)	2789403..2789762	+	360	WP_000581200.1	holo-ACP synthase	-
IF767_RS13950 (2789828)	2789828..2790976	+	1149	WP_001281145.1	alanine racemase	-
IF767_RS13955 (2791061)	2791061..2791231	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF767_RS13960 (2791228)	2791228..2791590	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF767_RS13965 (2791940)	2791940..2792941	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF767_RS13970 (2793060)	2793060..2793386	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF767_RS13975 (2793388)	2793388..2793867	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF767_RS13980 (2793842)	2793842..2794612	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174307 WP_000621175.1 NZ_CP062324:2791228-2791590 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174307 NZ_CP080760:c1239162-1238416 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174307 WP_000948331.1 NZ_CP062324:2791061-2791231 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174307 NZ_CP080760:c1238415-1238245 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1