Detailed information of TA system TA174294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 165135..165911
• Replicon: chromosome
• Accession: NZ_CP062324
• Organism: Staphylococcus aureus strain NAS_OP_063

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF767_RS01140
• Protein ID: WP_000031108.1
• Coordinates: 165759..165911 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF767_RS01135
• Protein ID: WP_001251224.1
• Coordinates: 165135..165734 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF767_RS01115 (161051)	161051..162508	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF767_RS01120 (162501)	162501..163223	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF767_RS01125 (163374)	163374..164501	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF767_RS01130 (164506)	164506..164976	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF767_RS01135 (165135)	165135..165734	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF767_RS01140 (165759)	165759..165911	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF767_RS01145 (166688)	166688..167083	+	396	WP_000901021.1	hypothetical protein	-
IF767_RS01150 (167279)	167279..168664	+	1386	WP_000116224.1	class II fumarate hydratase	-
IF767_RS01155 (169127)	169127..169948	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174294 WP_000031108.1 NZ_CP062324:165759-165911 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174294 NZ_CP080633:1957195-1957494 [Shewanella putrefaciens]
ATGGCTGAAGTGATCTGGACAGAGCCAGCCCTTCAAGAACTTGATGCCATTGCTGAGTATATTGCTTTGGACAACCCTGC
TGCTGCGAGCGATCTCGTGAAGGCAGTTTTCGACAAGACAGAACGTTTGGAGAATTTCCCCAAATCCGGGCGGATTCCTC
CTGAGCTCCCGGATTCGGTATACAGAGAGGTAGTGATTCCACCCTGTCGCATTTTTTATCGTGAGGATGAGCAGCGGGTT
TTCGTCCTCTTTGTCATGCGAGAGGAGCGACAGCTTCGTGCGTTCATGCTTGGAAACTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT174294 WP_001251224.1 NZ_CP062324:165135-165734 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174294 NZ_CP080633:1956950-1957207 [Shewanella putrefaciens]
ATGAAAGTTGAGCTAGTTACGAATCTAAAGCGTCAAGCGACAAAGATTCTGGCAGATCTGCACTTGTCGAAAGAGCCCGT
ATTGATTACAGAGCATGGTCAGCCATCTGCATACCTTGTAGATGTTCAAGATTATGAATTCATGCAGCGTCGGCTTGCGC
TGCTTGAGGGGCTCGCTCGTGGAGAGCGTGCTGTACTTGAGGGAAGAACGTATAGCCAAAGTGAAGCCAGAGATAAGATG
ACTAAATGGCTGAAGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4