Detailed information of TA system TA174290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2703379..2703908
• Replicon: chromosome
• Accession: NZ_CP062322
• Organism: Staphylococcus aureus strain NAS_OP_071

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF768_RS13415
• Protein ID: WP_000621175.1
• Coordinates: 2703546..2703908 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF768_RS13410
• Protein ID: WP_000948331.1
• Coordinates: 2703379..2703549 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF768_RS13380	2698415..2698975	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF768_RS13385	2699184..2699663	+	480	WP_001287079.1	hypothetical protein	-
IF768_RS13390	2699656..2701239	+	1584	WP_001294620.1	PH domain-containing protein	-
IF768_RS13395	2701226..2701717	+	492	WP_001205912.1	PH domain-containing protein	-
IF768_RS13400	2701721..2702080	+	360	WP_000581197.1	holo-ACP synthase	-
IF768_RS13405	2702146..2703294	+	1149	WP_001281154.1	alanine racemase	-
IF768_RS13410	2703379..2703549	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF768_RS13415	2703546..2703908	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF768_RS13420	2704258..2705259	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF768_RS13425	2705378..2705704	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF768_RS13430	2705706..2706185	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF768_RS13435	2706160..2706930	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174290 WP_000621175.1 NZ_CP062322:2703546-2703908 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174290 NZ_CP080633:c391838-391539 [Shewanella putrefaciens]
ATGAAAAATAAACGCTATAAACTGAGTAAGTTAGCTCAGACTCATTTACTAAAGATTAAAGACTACACCATTCAACACTT
TTCTGAGTCGCAGTGGCACAAATACAAACAAAGCCTAATATCCGGCTTGAAAATGTTAGCCGATAACCCCGGATTAGGCA
GAAGCTGCGACGATATTTACCCCAACGGATTCTACTTTCCTATTGGTAAACACACCGCCTACTTCACAAAAGAAGACGAC
TTCATCCTTATCGTTGCCCTGTTAGGTCAACCACAACTACCCCAAAACCACCTGAAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174290 WP_000948331.1 NZ_CP062322:2703379-2703549 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174290 NZ_CP080633:c392089-391847 [Shewanella putrefaciens]
GTGCAAACATTAACAGCCAATGACGCCAAACGAAATTTCGGTGAGTTACTGCTAAACGCTCAACGCGAACCCATCAAGAT
CAGCAAAAACAGTAAAGATGCGGTTGTCGTGATGTCTATCCACGACTTCGAACAGCTTGAGGCGATGAAAGCGGATTACC
TTAAACACTGCTTCAACGCTGCTAAAGAAGACTTAGCGCAAGGTAATGTTGTTGATGGTGAAGATTTTCTTAACGCCCTG
TAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1