Detailed information of TA system TA174264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1794801..1795330
• Replicon: chromosome
• Accession: NZ_CP062320
• Organism: Staphylococcus aureus strain NAS_OP_101

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF771_RS09095
• Protein ID: WP_000621175.1
• Coordinates: 1794801..1795163 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF771_RS09100
• Protein ID: WP_000948331.1
• Coordinates: 1795160..1795330 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF771_RS09075 (1791779)	1791779..1792549	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
IF771_RS09080 (1792524)	1792524..1793003	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF771_RS09085 (1793005)	1793005..1793331	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IF771_RS09090 (1793450)	1793450..1794451	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF771_RS09095 (1794801)	1794801..1795163	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF771_RS09100 (1795160)	1795160..1795330	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF771_RS09105 (1795415)	1795415..1796563	-	1149	WP_001281145.1	alanine racemase	-
IF771_RS09110 (1796629)	1796629..1796988	-	360	WP_000581200.1	holo-ACP synthase	-
IF771_RS09115 (1796992)	1796992..1797483	-	492	WP_001205910.1	PH domain-containing protein	-
IF771_RS09120 (1797470)	1797470..1799053	-	1584	WP_001294626.1	PH domain-containing protein	-
IF771_RS09125 (1799046)	1799046..1799525	-	480	WP_001287088.1	hypothetical protein	-
IF771_RS09130 (1799733)	1799733..1800293	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174264 WP_000621175.1 NZ_CP062320:c1795163-1794801 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174264 NZ_CP080620:3646193-3646489 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174264 WP_000948331.1 NZ_CP062320:c1795330-1795160 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174264 NZ_CP080620:3646491-3646886 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1