Detailed information of TA system TA174235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2784930..2785459
• Replicon: chromosome
• Accession: NZ_CP062314
• Organism: Staphylococcus aureus strain NAS_OP_133

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF776_RS13860
• Protein ID: WP_000621175.1
• Coordinates: 2785097..2785459 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF776_RS13855
• Protein ID: WP_000948331.1
• Coordinates: 2784930..2785100 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF776_RS13825 (2779967)	2779967..2780527	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF776_RS13830 (2780735)	2780735..2781214	+	480	WP_001287088.1	hypothetical protein	-
IF776_RS13835 (2781207)	2781207..2782790	+	1584	WP_001294626.1	PH domain-containing protein	-
IF776_RS13840 (2782777)	2782777..2783268	+	492	WP_001205910.1	PH domain-containing protein	-
IF776_RS13845 (2783272)	2783272..2783631	+	360	WP_000581200.1	holo-ACP synthase	-
IF776_RS13850 (2783697)	2783697..2784845	+	1149	WP_001281145.1	alanine racemase	-
IF776_RS13855 (2784930)	2784930..2785100	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF776_RS13860 (2785097)	2785097..2785459	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF776_RS13865 (2785809)	2785809..2786810	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF776_RS13870 (2786929)	2786929..2787255	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF776_RS13875 (2787257)	2787257..2787736	+	480	WP_031907373.1	anti-sigma B factor RsbW	-
IF776_RS13880 (2787711)	2787711..2788481	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T174235 WP_000621175.1 NZ_CP062314:2785097-2785459 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T174235 NZ_CP080594:c3718131-3717844 [Cronobacter sakazakii]
ATGAGCTATAAACTGGTCTTTGACCCACGTGCGCTGAAGGAGTGGCACAAACTGGGCGAGACCGTTAAAGCGCAATTCAA
AAAGAAGCTGGCGCACGTGCTGGCGGCTCCCAGGGTGAAATCCGCCCGGTTAAGTGGCCTGCCGGACTGCTACAAAATTA
AATTAAGAACGAGCGGTTACCGGCTGGTGTATCAGGTGCGCGATGACGCTGTCTGTGTGCTGGTCATCGCGATCGGCAAA
CGCGAAAACTTGACCGTATACCAGGGTGCCGGGCACCGTCTGGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT174235 WP_000948331.1 NZ_CP062314:2784930-2785100 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT174235 NZ_CP080594:c3718363-3718121 [Cronobacter sakazakii]
ATGGCCACAATCAACGTAAGGCTTGATGACAAGCTTAAAAATGAGGCTTATGCGGTGCTGGAAAAACTGGGTATCACACC
TACCGAAGCGGTGCGTCTGCTGTTTCAGTATGTGGCACAAAATGGCCGTATGCCGGTCAAAACGCTAACCGTCAGCGACA
GCGAAGAGGCGCTGCTGCGTACCGTGCGTGAGCGACTGGCGGACCCGCGGCCGGGCATCAGGGTTGATCTGGATGAGCTA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1