Detailed information of TA system TA174234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2765189..2765703
• Replicon: chromosome
• Accession: NZ_CP062314
• Organism: Staphylococcus aureus strain NAS_OP_133

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF776_RS13770
• Protein ID: WP_000581792.1
• Coordinates: 2765494..2765703 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: IF776_RS13765
• Protein ID: WP_000138932.1
• Coordinates: 2765189..2765482 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF776_RS13745 (2760552)	2760552..2761193	+	642	WP_000483153.1	thiamine phosphate synthase	-
IF776_RS13750 (2761279)	2761279..2762151	+	873	WP_000725802.1	membrane protein insertase YidC	-
IF776_RS13755 (2762864)	2762864..2763511	-	648	WP_001187629.1	HD domain-containing protein	-
IF776_RS13760 (2763539)	2763539..2765023	-	1485	WP_000571549.1	cardiolipin synthase	-
IF776_RS13765 (2765189)	2765189..2765482	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF776_RS13770 (2765494)	2765494..2765703	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF776_RS13775 (2765831)	2765831..2765968	+	138	WP_000828354.1	Lmo0850 family protein	-
IF776_RS13780 (2766232)	2766232..2767434	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF776_RS13785 (2767752)	2767752..2768822	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF776_RS13790 (2768837)	2768837..2770195	+	1359	WP_000611462.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T174234 WP_000581792.1 NZ_CP062314:2765494-2765703 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T174234 NZ_CP080594:c3629828-3629244 [Cronobacter sakazakii]
ATGTCCATTGATAACGATCTGTTCGAAAGCATACTTAGAGGCGCTATCAGCGCTAATATTGACGATCTTGTTAAAACTCT
TACTTCCACTGAGCCGCAAAATTCTTCCACCCCCCCTCCGCAAGAAGAAATTGATGGGGCAGACGACAAAGCCCAAAAAG
AAGCGCAAGCCTTTGATACACCAACAGAAGATGAGCATGAAGAACTTCAAGCGCTAGGGTATTGGAGCTTATCGCATCAA
AGACAAGTTGACTCCCTAACCATACAAAAATCGAAAGAAGAAATTGTTCGGATTAGCTGCGAAAACGAAGAGTTAAGGGC
AAAAACTAAGAATAATCACGCTATGGCTAGAGGACGACGTATCGATAATCGATTACGTCTGCAAATGGCTGCTGCTACCT
TCCGCTTTATGCAATATTGGTGTTGTTTTGTTGCATTAGTGGTTTTCATTTATATAGCAAAAAATGAAGGCAACCCTCCT
AGTGAAGTGATAATTGCGCTACTCGGTACAAGCACCATTAGCATTGTTGGTTTGGTTGGTTTTGTTGTCAGTGGATTATT
TAAAAGCAACAAAGATAAAGAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT174234 WP_000138932.1 NZ_CP062314:2765189-2765482 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT174234 NZ_CP080594:c3630340-3629870 [Cronobacter sakazakii]
ATGTATAACCCGATTTGTATCGTTAATGAATTTATTGAGCGAGCAAAGAAAAAGGGGACTGACCTCACTCACATGCAGGC
CCAGAAACTGCTGTACATAGCTCATGGCTATAGCTTAGCGTTTCTAAATGAGCCCTTGCTTGAAGAACCCGTGTGTGCGT
GGAGGTATGGTCCGGTCATACCGTCCGTGTATTATGCTCTGCGTCACAATCGTTCTGAACCGATTGCAGATAAAGCGCCG
ACAACTGCAATTGAAACAGAAACCTTAGATAGCAAAACAGCTTCTTTGCTTGATATGGTTTACGAAACTTACGGTAAGTT
ATCTGGAGTTGTTCTGTCTGAGTTTACTCATCGTCCTGGCACGCCGTGGTCACAAGCAATGGGGAAAAGAGAAAATATAA
TCAGTGACTCGAGTATTAAAGAATACTATCTAAAACTGATAAAACGTGACCCGGTTTGCAACGGATTGTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13