Detailed information of TA system TA174222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 121495..122271
• Replicon: chromosome
• Accession: NZ_CP062314
• Organism: Staphylococcus aureus strain NAS_OP_133

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF776_RS00780
• Protein ID: WP_000031108.1
• Coordinates: 122119..122271 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF776_RS00775
• Protein ID: WP_001251224.1
• Coordinates: 121495..122094 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF776_RS00755 (117411)	117411..118868	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF776_RS00760 (118861)	118861..119583	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF776_RS00765 (119734)	119734..120861	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF776_RS00770 (120866)	120866..121336	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF776_RS00775 (121495)	121495..122094	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF776_RS00780 (122119)	122119..122271	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF776_RS00785 (123048)	123048..123443	+	396	WP_000901021.1	hypothetical protein	-
IF776_RS00790 (123639)	123639..125024	+	1386	WP_000116224.1	class II fumarate hydratase	-
IF776_RS00795 (125487)	125487..126308	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T174222 WP_000031108.1 NZ_CP062314:122119-122271 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T174222 NZ_CP080591:c3662224-3661937 [Cronobacter sakazakii]
ATGAGCTATAAACTGGTCTTTGACCCACGTGCGCTGAAGGAGTGGCACAAACTGGGCGAGACCGTTAAAGCGCAATTCAA
AAAGAAGCTGGCGCACGTGCTGGCGGCTCCCAGGGTGAAATCCGCCTGGTTAAGTGGCCTGCCGGACTGCTACAAAATTA
AATTAAGAACGAGCGGTTACCGGCTGGTGTATCAGGTGCGCGATGATGCTGTCTGTGTGCTGGTCATCGCGATCGGCAAA
CGCGAAAACCTGACCGTATACCAGGGTGCCGGGCACCGTCTGGAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT174222 WP_001251224.1 NZ_CP062314:121495-122094 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT174222 NZ_CP080591:c3662456-3662214 [Cronobacter sakazakii]
ATGGCCACAATCAACGTAAGGCTTGATGACAAGCTTAAAAATGAGGCTTATGCGGTGCTGGAAAAACTAGGTATCACCCC
TACCGAAGCGGTGCGTCTGCTGTTTCAGTATGTGGCGCAAAATGGCCGTATGCCGGTCAAAACGCTAACCGTCAGCGACA
GCGAAGAGGCGCTGCTGCGTACCGTGCGGGAGCGACTGGCGGATCCGCAGCCGGGCATCAGGGTTGATCTGGATGAGCTA
TAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4