Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 29595..30732 Replicon plasmid pVB3338_P3
Accession NZ_CP062268
Organism Enterococcus faecium strain VB3338

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag IG632_RS15185 Protein ID WP_001284311.1
Coordinates 29595..30458 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag IG632_RS15190 Protein ID WP_000301765.1
Coordinates 30460..30732 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG632_RS15155 25641..25892 + 252 Protein_34 GNAT family N-acetyltransferase -
IG632_RS15160 25985..26779 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
IG632_RS15165 27139..28098 + 960 WP_002303350.1 IS30 family transposase -
IG632_RS15170 28255..28338 + 84 Protein_37 peptide-binding protein -
IG632_RS15175 28387..28470 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
IG632_RS15180 28595..29332 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
IG632_RS15185 29595..30458 - 864 WP_001284311.1 toxin zeta Toxin
IG632_RS15190 30460..30732 - 273 WP_000301765.1 antitoxin Antitoxin
IG632_RS15195 30749..30958 - 210 WP_000527318.1 peptide-binding protein -
IG632_RS15200 31056..31313 - 258 WP_002347171.1 hypothetical protein -
IG632_RS15205 31369..32049 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
IG632_RS15210 32125..32709 + 585 Protein_45 IS6-like element IS1216 family transposase -
IG632_RS15215 32764..33342 + 579 Protein_46 IS30 family transposase -
IG632_RS15220 33366..33662 - 297 WP_000053907.1 replication control protein PrgN -
IG632_RS15225 33797..35290 - 1494 WP_000947691.1 primase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / erm(B) - 1..43862 43862
- inside IScluster/Tn aph(3')-III / erm(B) - 23355..42334 18979


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T174195 WP_001284311.1 NZ_CP062268:c30458-29595 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T174195 NZ_CP080584:745520-746005 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT174195 WP_000301765.1 NZ_CP062268:c30732-30460 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT174195 NZ_CP080584:745230-745523 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References