Detailed information of TA system TA174194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 9852..10459
• Replicon: plasmid pVB3338_P3
• Accession: NZ_CP062268
• Organism: Enterococcus faecium strain VB3338

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: IG632_RS15055
• Protein ID: WP_002321032.1
• Coordinates: 9852..10202 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: IG632_RS15060
• Protein ID: WP_002287514.1
• Coordinates: 10196..10459 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG632_RS15030	6138..6722	-	585	WP_002299575.1	recombinase family protein	-
IG632_RS15035	6933..7136	-	204	WP_002299573.1	hypothetical protein	-
IG632_RS15040	7146..7568	-	423	WP_002303116.1	hypothetical protein	-
IG632_RS15045	7703..8875	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
IG632_RS15050	9214..9828	+	615	WP_010729795.1	recombinase family protein	-
IG632_RS15055	9852..10202	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IG632_RS15060	10196..10459	-	264	WP_002287514.1	hypothetical protein	Antitoxin
IG632_RS15065	10800..10979	-	180	WP_002305761.1	hypothetical protein	-
IG632_RS15070	11360..12067	-	708	WP_002353391.1	hypothetical protein	-
IG632_RS15075	12060..12497	-	438	WP_002296237.1	hypothetical protein	-
IG632_RS15080	12512..12763	-	252	WP_002296238.1	hypothetical protein	-
IG632_RS15085	12763..12969	-	207	WP_002296239.1	hypothetical protein	-
IG632_RS15090	13410..13670	-	261	WP_002296240.1	hypothetical protein	-
IG632_RS15095	14036..14521	-	486	WP_002296241.1	single-stranded DNA-binding protein	-
IG632_RS15100	14524..15423	-	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / erm(B)	-	1..43862	43862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T174194 WP_002321032.1 NZ_CP062268:c10202-9852 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T174194 NZ_CP080584:355393-355740 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT174194 WP_002287514.1 NZ_CP062268:c10459-10196 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT174194 NZ_CP080584:355740-356039 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0