Detailed information of TA system TA174192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 18210..18729
• Replicon: plasmid pVB3338_P1
• Accession: NZ_CP062266
• Organism: Enterococcus faecium strain VB3338

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: IG632_RS13585
• Protein ID: WP_000588503.1
• Coordinates: 18472..18729 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: IG632_RS13580
• Protein ID: WP_000388479.1
• Coordinates: 18210..18479 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG632_RS13535	13407..14306	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
IG632_RS13540	14309..14794	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
IG632_RS13545	14935..15138	-	204	WP_002316074.1	putative holin-like toxin	-
IG632_RS13550	15213..15473	+	261	WP_002295623.1	hypothetical protein	-
IG632_RS13555	15910..16116	+	207	WP_002347218.1	hypothetical protein	-
IG632_RS13560	16116..16367	+	252	WP_002296238.1	hypothetical protein	-
IG632_RS13565	16382..16819	+	438	WP_002300851.1	hypothetical protein	-
IG632_RS13570	16812..17519	+	708	WP_002303117.1	hypothetical protein	-
IG632_RS13575	17900..18079	+	180	WP_001120991.1	hypothetical protein	-
IG632_RS13580	18210..18479	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IG632_RS13585	18472..18729	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
IG632_RS13590	19188..19343	+	156	WP_002347142.1	hypothetical protein	-
IG632_RS13595	19367..20191	+	825	WP_001809248.1	hypothetical protein	-
IG632_RS13600	20356..20970	-	615	WP_001261742.1	recombinase family protein	-
IG632_RS13605	21208..21630	+	423	WP_002303116.1	hypothetical protein	-
IG632_RS13610	21640..21843	+	204	WP_002299573.1	hypothetical protein	-
IG632_RS13615	22054..22638	+	585	WP_002299575.1	recombinase family protein	-
IG632_RS13620	22827..23507	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..227172	227172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T174192 WP_000588503.1 NZ_CP062266:18472-18729 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T174192 NZ_CP080577:128231-128521 [Acinetobacter lwoffii]
ATGCGGACGATTAAGTACACATCAGCATTCAAGAAAGACTATAAGCGTGAGAAAAAAGGGCAGCATCGAAATACAGTAGA
TGCTGAACTACAAACTGTTTTAGAGTTATTGCAAGCTGATGAAACCTTGCCTGAAAAGTATCGTGACCATGCTTTGGTCA
GTAATTGGGTAGGTCATCGTGACTGCCATATCAAACCAGATCTTGTTTTGATCTATAAAAAAACAGAGGAAGGAGAACTA
CTAATTTTGAAATTAGTTCGGTTAGGTAGTCATAGTGAATTAGCGATTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT174192 WP_000388479.1 NZ_CP062266:18210-18479 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT174192 NZ_CP080577:127984-128244 [Acinetobacter lwoffii]
ATGGCTTCAAATGCACTAGTAACTACTCGCATTAATGAAAACATTAAGCATGAAGCAACAGAAGTTCTTGCAACAATGGG
CTTAACTGTCTCAGATGCAGTTCGCTTGTTGTTAACCAAAGTCGCGAAAGAAAAGGCCCTGCCTTTTGAAATTTGGCAAC
CCAGTGCTGAAACCATTGCTGCAATGGAAGAAGATCTAAGCAAAGCAAAGAGCTTCACTTCTGTAAAAGATTTGATGGCT
GATCTAAATGCGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7