Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1814864..1815435 Replicon chromosome
Accession NZ_CP062265
Organism Enterococcus faecium strain VB3338

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag IG632_RS08675 Protein ID WP_002286801.1
Coordinates 1815094..1815435 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag IG632_RS08670 Protein ID WP_002323011.1
Coordinates 1814864..1815094 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG632_RS08640 1809997..1811328 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
IG632_RS08645 1811350..1811976 + 627 WP_002286816.1 cysteine hydrolase -
IG632_RS08650 1812159..1812740 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
IG632_RS08655 1813472..1813819 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
IG632_RS08660 1813795..1814034 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
IG632_RS08665 1814239..1814577 - 339 WP_002286804.1 hypothetical protein -
IG632_RS08670 1814864..1815094 + 231 WP_002323011.1 hypothetical protein Antitoxin
IG632_RS08675 1815094..1815435 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IG632_RS08680 1816285..1816470 + 186 WP_002304207.1 hypothetical protein -
IG632_RS08685 1816975..1817169 + 195 WP_002297028.1 hypothetical protein -
IG632_RS08690 1817327..1817609 + 283 Protein_1671 transposase -
IG632_RS08695 1817853..1818683 - 831 WP_002286789.1 manganese catalase family protein -
IG632_RS08700 1818891..1820225 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T174191 WP_002286801.1 NZ_CP062265:1815094-1815435 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T174191 NZ_CP080576:c951335-950943 [Acinetobacter lwoffii]
ATGGATAATCGTTGCTTCAAGCTGAAACGCAGTCTGTGTGCATTGGCGTTTCAGCTTTTTATTTTTGCACTGCTGATGTA
TCTGTTGTATCAGTTATTGCCGATTGCACTTTGGGCAGTGTGTGCTGTCATAGGCTTGCTGATTTATCAGCTGTTTTACC
GCAAGACTCCCCAGATAGAGCAGTTTGAATATCTGGATGGGCGTGAATGGTCATTGACCGCCAAAGCACGACCGACCCGC
CGGGTATGTATTAGTCACGTGATTGATCATCAAGCGTATATTGTGGTGTATTTCCAGCATGCCAAGGCAAGACCTTTACT
GATCTGGTGTGATCAATTGCCCTTCAAACAATGGAAATCCTTAAAATTATTGACTAAATTGATTGAGTTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT174191 WP_002323011.1 NZ_CP062265:1814864-1815094 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT174191 NZ_CP080576:c951585-951328 [Acinetobacter lwoffii]
ATGACTGATGACATTAGCTTGGAAGAGCGCAAAGTGATTTATCGTGCGCGTCGTGGTCTAAAAGAACTGGATGTATATTT
TGATCCCTACGTCAAAAACTACTACCTGAATGCCGAGCCTTTCGAAAAGCAGATGTTTGCAGAGCTGGTGGAGCAGGAAG
ACCCGGATTTGCTGGACTGGTTTATGGAAGTATCCGAGCCGCCGCGTCCTGAACTGAAAGATTTTATCAAGAAGCTTAAA
TTTTACGTTCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References