Detailed information of TA system TA174189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 6872990..6873616
• Replicon: chromosome
• Accession: NZ_CP062260
• Organism: Paenibacillus sp. JNUCC-32

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4ARZ2
• Locus tag: JNUCC32_RS30665
• Protein ID: WP_006212388.1
• Coordinates: 6873266..6873616 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: JNUCC32_RS30660
• Protein ID: WP_006212389.1
• Coordinates: 6872990..6873262 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNUCC32_RS30640	6868026..6868526	+	501	WP_192570687.1	winged helix-turn-helix domain-containing protein	-
JNUCC32_RS30645	6868589..6869917	+	1329	WP_192570688.1	MFS transporter	-
JNUCC32_RS30650	6870181..6871293	+	1113	WP_096776514.1	outer membrane lipoprotein carrier protein LolA	-
JNUCC32_RS30655	6871566..6872753	+	1188	WP_192570689.1	alanine racemase	-
JNUCC32_RS30660	6872990..6873262	+	273	WP_006212389.1	ribbon-helix-helix protein, CopG family	Antitoxin
JNUCC32_RS30665	6873266..6873616	+	351	WP_006212388.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JNUCC32_RS30670	6874060..6876255	+	2196	WP_192572757.1	RNA-binding transcriptional accessory protein	-
JNUCC32_RS30675	6876461..6877021	-	561	WP_192570690.1	CGNR zinc finger domain-containing protein	-
JNUCC32_RS30680	6877166..6877636	+	471	WP_192570691.1	DinB family protein	-
JNUCC32_RS30685	6877734..6877865	-	132	WP_015737250.1	cortex morphogenetic protein CmpA	-
JNUCC32_RS30690	6877946..6878296	-	351	WP_009594449.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12751.64 Da
• Isoelectric Point: 4.8265

>T174189 WP_006212388.1 NZ_CP062260:6873266-6873616 [Paenibacillus sp. JNUCC-32]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T174189 NZ_CP080573:c249051-248743 [Stenotrophomonas maltophilia]
ATGAGCAAGCTGAAGACGATTCCCTTCGATCCCGCCGACTATCTGGATGATGACGTCGCAATCGCCACCTATCTGAGCGA
GGCCTTGGCAGCAGGTGATGCGGGCCACTTCCAAGAGGCACTCGGAACAGTGGCACGTGCCCGTGGCATGGGCGGCGTTG
CCGAGGTCTCCGGTCTTGGACGCGAGAGCCTGTACAAGGCGCTGCGCCCCGGAGCACACCCACGCTTCGACACCGTGCAG
CGGGTACTGTCAGCGCTCGGGGTGCATCTCATCGTGCAAGCCGACAAGCAACCCGCTGGCACCGCCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10500.04 Da
• Isoelectric Point: 6.4708

>AT174189 WP_006212389.1 NZ_CP062260:6872990-6873262 [Paenibacillus sp. JNUCC-32]
MQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
NTLDRLVSGV

Nucleotide

• Length: 273 bp

>AT174189 NZ_CP080573:c249380-249090 [Stenotrophomonas maltophilia]
ATGGCAACCCTCCAGGCAACAGAGACGTTCACGCGCTGGCTGCGCGGGCTCAATAACAGCGGGACCCGGGCCGTGATCGT
GGAACGCCTGCAGCGCGTCGCCCGCGGTCTCGAGGGAGATGTTCGATCGGTGGGTCATGGCGTGAGCGAACTGCGTATCA
ACCATGGACCGGGATATCGCGTGTATTTCACACGCCGCGGGAGATCCATCGTCGTGCTCCTGCACGGTGGCGACAAGTCG
AGCCAGCGTCGCGATATCGAGAAAGCGGTCAGGCTCGCGGAGTTGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A1S2EZN8