Detailed information of TA system TA174188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 18225..18814
• Replicon: plasmid pXAC33
• Accession: NZ_CP062257
• Organism: Xanthomonas sp. gxlp16

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: IG630_RS22540
• Protein ID: WP_015463824.1
• Coordinates: 18225..18557 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: IG630_RS22545
• Protein ID: WP_011052949.1
• Coordinates: 18557..18814 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG630_RS22520	16705..16926	-	222	WP_011052944.1	hypothetical protein	-
IG630_RS22525	17216..17752	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
IG630_RS22530	17742..17954	-	213	WP_011052947.1	hypothetical protein	-
IG630_RS22535	17951..18202	-	252	WP_125459571.1	hypothetical protein	-
IG630_RS22540	18225..18557	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IG630_RS22545	18557..18814	-	258	WP_011052949.1	antitoxin	Antitoxin
IG630_RS22550	19020..19622	+	603	WP_011052950.1	recombinase family protein	-
IG630_RS22555	19606..19910	+	305	Protein_28	Tn3 family transposase	-
IG630_RS22560	20077..20283	-	207	Protein_29	Tn3 family transposase	-
IG630_RS22565	20328..20896	-	569	Protein_30	UPF0149 family protein	-
IG630_RS22570	20915..22129	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
IG630_RS22575	22203..22385	-	183	Protein_32	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33698	33698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T174188 WP_015463824.1 NZ_CP062257:c18557-18225 [Xanthomonas sp. WG16]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T174188 NZ_CP080566:c2628931-2628665 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT174188 WP_011052949.1 NZ_CP062257:c18814-18557 [Xanthomonas sp. WG16]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT174188 NZ_CP080566:c2629188-2628931 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6