Detailed information of TA system TA174186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 31393..31934
• Replicon: plasmid pXAC64
• Accession: NZ_CP062256
• Organism: Xanthomonas sp. gxlp16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IG630_RS22225
• Protein ID: WP_015462614.1
• Coordinates: 31393..31719 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: IG630_RS22230
• Protein ID: WP_003491150.1
• Coordinates: 31716..31934 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG630_RS22215	26776..28350	+	1575	WP_011052977.1	type IV secretion system DNA-binding domain-containing protein	-
IG630_RS22220	28364..31339	+	2976	WP_011052978.1	conjugative relaxase	-
IG630_RS22225	31393..31719	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IG630_RS22230	31716..31934	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
IG630_RS22235	32071..32457	-	387	WP_003491149.1	hypothetical protein	-
IG630_RS22240	32534..32953	-	420	WP_011052981.1	hypothetical protein	-
IG630_RS22245	33079..33957	-	879	WP_011052982.1	transglycosylase SLT domain-containing protein	-
IG630_RS22250	33968..34990	-	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-
IG630_RS22255	35001..36221	-	1221	WP_011052984.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64919	64919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T174186 WP_015462614.1 NZ_CP062256:c31719-31393 [Xanthomonas sp. WG16]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T174186 NZ_CP080566:c2572424-2572158 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT174186 WP_003491150.1 NZ_CP062256:c31934-31716 [Xanthomonas sp. WG16]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT174186 NZ_CP080566:c2572675-2572424 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: H8FMA7