Detailed information of TA system TA174157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Acetyltransf_9(toxin)
• Location: 2852893..2854603
• Replicon: chromosome
• Accession: NZ_CP062252
• Organism: Pseudomonas allokribbensis strain IzPS23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IF199_RS12975
• Protein ID: WP_192560649.1
• Coordinates: 2852893..2853960 (-)
• Length: 356 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IF199_RS12980
• Protein ID: WP_096821653.1
• Coordinates: 2854046..2854603 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF199_RS12950	2848351..2848983	-	633	WP_085708430.1	DUF1842 domain-containing protein	-
IF199_RS12955	2849018..2849203	-	186	WP_085708738.1	DUF1843 domain-containing protein	-
IF199_RS12960	2849753..2850670	+	918	WP_192560646.1	LysR family transcriptional regulator	-
IF199_RS12965	2850708..2851316	-	609	WP_192560647.1	3-isopropylmalate dehydratase small subunit	-
IF199_RS12970	2851320..2852741	-	1422	WP_192560648.1	3-isopropylmalate dehydratase large subunit	-
IF199_RS12975	2852893..2853960	-	1068	WP_192560649.1	GNAT family N-acetyltransferase	Toxin
IF199_RS12980	2854046..2854603	-	558	WP_096821653.1	helix-turn-helix domain-containing protein	Antitoxin
IF199_RS12985	2854682..2855086	-	405	WP_192560650.1	RidA family protein	-
IF199_RS12990	2855221..2856162	+	942	WP_192560651.1	LysR family transcriptional regulator	-
IF199_RS12995	2856125..2856985	-	861	WP_096821656.1	LysR family transcriptional regulator	-
IF199_RS13000	2857123..2858175	+	1053	WP_192560652.1	agmatine deiminase family protein	-
IF199_RS13005	2858179..2859087	+	909	WP_192560653.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (9-164)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-53)

Sequences

Toxin

Protein

• Length: 356 a.a.
• Molecular weight: 39199.31 Da
• Isoelectric Point: 4.9594

>T174157 WP_192560649.1 NZ_CP062252:c2853960-2852893 [Pseudomonas allokribbensis]
MNLEKIKGRLDFLREAERLKDVLRSAHTSSGRNESTAEHSWRLCLMAILFADDLAGLDVLKVLKMCVIHDLGEAINGDIP
AVNKADFPDKGEQERNDLLLLTRSLDDALRNEILVLWDDYENARSPEAKAVKALDKLETLLQHTQGKNPPDFDYGFNLAY
GKQYTNADPLFETLRTLIDRDTRERMNTNITLRNERPEDIDAIARITEAAFQHEEHSSHTEQFIVNALRRAGQLSVSLVA
VENDTVVGHVAISPVTISSGTQDWYGLGPISVCPTRQQQGIGSALMKASLAELQRIGGVGCVVLGDPGYYGRFGFKAHAG
LELPGIPAEYFQALAFGGELPVGVVKYHEAFDATA

Nucleotide

• Length: 1068 bp

>T174157 NZ_CP080562:c878472-878284 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20543.55 Da
• Isoelectric Point: 7.8760

>AT174157 WP_096821653.1 NZ_CP062252:c2854603-2854046 [Pseudomonas allokribbensis]
MPDTLLKPSLPGIALRRWRVLHRVKQSHAAELFKVTQSTISRWESGMQAMDPEAHRQLETLLAARLDSAADQALARLVTD
SARPVHLVCDLTHRLLACSPARAAQFSSPLSDLLGQSLWRFATAQIQHKESLLDDAGWREVQAPPALEFVTGHNDSTLVP
IRESLCRWTRIALSDGTAARLVETL

Nucleotide

• Length: 558 bp

>AT174157 NZ_CP080562:c878761-878501 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA