Detailed information of TA system TA174156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1539338..1539961
• Replicon: chromosome
• Accession: NZ_CP062252
• Organism: Pseudomonas allokribbensis strain IzPS23

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IF199_RS07025
• Protein ID: WP_095049193.1
• Coordinates: 1539782..1539961 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IF199_RS07020
• Protein ID: WP_192560011.1
• Coordinates: 1539338..1539745 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF199_RS06985	1534406..1534945	-	540	WP_192560913.1	hypothetical protein	-
IF199_RS06990	1535069..1535572	-	504	WP_007957947.1	inclusion body family protein	-
IF199_RS06995	1535945..1536715	+	771	WP_192560007.1	trans-aconitate 2-methyltransferase	-
IF199_RS07000	1536756..1537484	-	729	WP_192560008.1	class I SAM-dependent methyltransferase	-
IF199_RS07005	1538008..1538376	+	369	WP_192560009.1	hypothetical protein	-
IF199_RS07010	1538457..1538816	-	360	WP_085683210.1	transcriptional regulator	-
IF199_RS07015	1538816..1539118	-	303	WP_192560010.1	type II toxin-antitoxin system HigB family toxin	-
IF199_RS07020	1539338..1539745	-	408	WP_192560011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IF199_RS07025	1539782..1539961	-	180	WP_095049193.1	type II toxin-antitoxin system HicA family toxin	Toxin
IF199_RS07035	1540646..1541530	-	885	WP_192560012.1	alpha/beta hydrolase	-
IF199_RS07040	1541846..1542538	-	693	WP_096819430.1	pseudouridine synthase	-
IF199_RS07045	1542572..1542790	-	219	WP_192560013.1	cysteine-rich CWC family protein	-
IF199_RS07050	1542795..1544282	-	1488	WP_096819429.1	sensor domain-containing diguanylate cyclase	-
IF199_RS07055	1544490..1544753	+	264	WP_192560014.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1529221..1539961	10740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6646.93 Da
• Isoelectric Point: 11.1923

>T174156 WP_095049193.1 NZ_CP062252:c1539961-1539782 [Pseudomonas allokribbensis]
VNSRFLIGQLVADGWYLVRIKGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 180 bp

>T174156 NZ_CP080560:c2645309-2645043 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14673.75 Da
• Isoelectric Point: 4.7269

>AT174156 WP_192560011.1 NZ_CP062252:c1539745-1539338 [Pseudomonas allokribbensis]
MLYPIAISMGDEGHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPKYAGCTWAL
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQR

Nucleotide

• Length: 408 bp

>AT174156 NZ_CP080560:c2645566-2645309 [Staphylococcus aureus]
ATGATTATCACTAGTCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA