Detailed information of TA system TA174155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 495908..496497
• Replicon: chromosome
• Accession: NZ_CP062252
• Organism: Pseudomonas allokribbensis strain IzPS23

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IF199_RS02225
• Protein ID: WP_192559576.1
• Coordinates: 495908..496213 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q3KJ92
• Locus tag: IF199_RS02230
• Protein ID: WP_011332089.1
• Coordinates: 496210..496497 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF199_RS02205	491938..493005	+	1068	WP_007952372.1	uroporphyrinogen decarboxylase	-
IF199_RS02210	493062..494336	-	1275	WP_192559575.1	MFS transporter	-
IF199_RS02215	494455..495351	+	897	WP_039766450.1	LysR family transcriptional regulator	-
IF199_RS02220	495352..495789	-	438	WP_096819910.1	excinuclease	-
IF199_RS02225	495908..496213	+	306	WP_192559576.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IF199_RS02230	496210..496497	+	288	WP_011332089.1	putative addiction module antidote protein	Antitoxin
IF199_RS02235	496546..496989	-	444	WP_192559577.1	excinuclease	-
IF199_RS02240	497010..498245	-	1236	WP_192559578.1	beta-ketoacyl-ACP synthase	-
IF199_RS02245	498245..498973	-	729	WP_192559579.1	3-oxoacyl-ACP reductase FabG	-
IF199_RS02250	498966..499430	-	465	WP_192559580.1	hotdog family protein	-
IF199_RS02255	499427..500623	-	1197	WP_192559581.1	beta-ketoacyl-[acyl-carrier-protein] synthase family protein	-
IF199_RS02260	500620..501102	-	483	WP_192559582.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11454.10 Da
• Isoelectric Point: 9.6535

>T174155 WP_192559576.1 NZ_CP062252:495908-496213 [Pseudomonas allokribbensis]
MTEILRSSTFSSWLVKLADSRARMRIQVRIDRMADGNFGDVKAIGEGISEARIDYGPGYRVYFMLQGRQLVILLCGGDKS
SQSRDIKQARLIAESWQEQNP

Nucleotide

• Length: 306 bp

>T174155 NZ_CP080560:c2588612-2588346 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10446.86 Da
• Isoelectric Point: 4.6412

>AT174155 WP_011332089.1 NZ_CP062252:496210-496497 [Pseudomonas allokribbensis]
MTEQFTRWDSAEYLKTEEDMANYLDACMEEAGDDPAFIAKALGTIARARGMTQVARDAGLSRESLYRALSGEVNPEFGTI
LKVMKALGLKLHAST

Nucleotide

• Length: 288 bp

>AT174155 NZ_CP080560:c2588863-2588612 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A345RJ97