Detailed information of TA system TA174154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 283704..284347
• Replicon: chromosome
• Accession: NZ_CP062252
• Organism: Pseudomonas allokribbensis strain IzPS23

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A345RIP1
• Locus tag: IF199_RS01215
• Protein ID: WP_065261145.1
• Coordinates: 283704..283886 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IF199_RS01220
• Protein ID: WP_170929726.1
• Coordinates: 283922..284347 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF199_RS01195	278798..279790	-	993	WP_096821893.1	D-cysteine desulfhydrase	-
IF199_RS01200	279981..280907	+	927	WP_096821894.1	serine O-acetyltransferase	-
IF199_RS01205	281196..283157	+	1962	WP_173861336.1	choline transporter BetT	-
IF199_RS01210	283182..283442	-	261	WP_096821896.1	hypothetical protein	-
IF199_RS01215	283704..283886	+	183	WP_065261145.1	type II toxin-antitoxin system HicA family toxin	Toxin
IF199_RS01220	283922..284347	+	426	WP_170929726.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IF199_RS01225	284370..285407	-	1038	WP_192559488.1	LacI family DNA-binding transcriptional regulator	-
IF199_RS01230	285614..287839	+	2226	WP_192559489.1	TonB-dependent receptor	-
IF199_RS01235	287841..289160	+	1320	WP_192559490.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-135)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6836.96 Da
• Isoelectric Point: 10.5417

>T174154 WP_065261145.1 NZ_CP062252:283704-283886 [Pseudomonas allokribbensis]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPGKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T174154 NZ_CP080560:c2271898-2271689 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15500.30 Da
• Isoelectric Point: 4.2513

>AT174154 WP_170929726.1 NZ_CP062252:283922-284347 [Pseudomonas allokribbensis]
MSEEGFTYMKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFDNTQEALALHYEGLVADGAPLPRVQDIDAHLENPDY
AGGIWGVVEFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 426 bp

>AT174154 NZ_CP080560:c2272203-2271910 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A345RIP1