Detailed information of TA system TA174150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Acetyltransf_9(toxin)
• Location: 2747994..2749704
• Replicon: chromosome
• Accession: NZ_CP062251
• Organism: Pseudomonas sp. IzPS59

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IF189_RS12240
• Protein ID: WP_134826573.1
• Coordinates: 2747994..2749061 (-)
• Length: 356 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IF189_RS12245
• Protein ID: WP_134826574.1
• Coordinates: 2749147..2749704 (-)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF189_RS12210	2743536..2744144	-	609	WP_134826567.1	3-isopropylmalate dehydratase small subunit	-
IF189_RS12215	2744148..2745569	-	1422	WP_134826568.1	3-isopropylmalate dehydratase large subunit	-
IF189_RS12220	2745705..2746190	-	486	WP_134826569.1	MaoC family dehydratase	-
IF189_RS12225	2746187..2746759	-	573	WP_192553395.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
IF189_RS12230	2746786..2747403	-	618	WP_134826571.1	LysE family translocator	-
IF189_RS12235	2747527..2747979	+	453	WP_134826572.1	Lrp/AsnC family transcriptional regulator	-
IF189_RS12240	2747994..2749061	-	1068	WP_134826573.1	GNAT family N-acetyltransferase	Toxin
IF189_RS12245	2749147..2749704	-	558	WP_134826574.1	helix-turn-helix domain-containing protein	Antitoxin
IF189_RS12250	2749783..2750187	-	405	WP_192553396.1	RidA family protein	-
IF189_RS12255	2750322..2751269	+	948	WP_192553397.1	LysR family transcriptional regulator	-
IF189_RS12260	2751339..2752499	+	1161	WP_134826577.1	radical SAM protein	-
IF189_RS12265	2752481..2753368	-	888	WP_134826578.1	LysR family transcriptional regulator	-
IF189_RS12270	2753506..2754558	+	1053	WP_192553398.1	agmatine deiminase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-172)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-53)

Sequences

Toxin

Protein

• Length: 356 a.a.
• Molecular weight: 38980.98 Da
• Isoelectric Point: 4.8062

>T174150 WP_134826573.1 NZ_CP062251:c2749061-2747994 [Pseudomonas sp. IzPS59]
MNLEKIKGRLDFLREAERLKDVLRSAHTSSGRSESTAEHSWRLCLMAIVFADDLAGLDMLKVLKMCVIHDLGEAINGDIP
AVDQAGFPDKGEQERNDLLLLTRSLDDALRSEILALWDDYENAGSPEAKAVKALDKLETLLQHTQGKNPPDFDYGFNLAY
GKQYTNADPLFETLRTLIDRDTRERMNTNITVRNERPEDIDAIARITEAAFQHEEHSSHTEQFIVNALRRAGQLSVSLVA
VENDTVVGHVAISPVTISSGTQGWYGLGPISVCPTRQQQGIGSALMKDSLAELQRIGGVGCVVLGDPGYYGRFGFKAHAG
LELPGIPAEYFQALAFGGELPIGVVSYHKAFDATE

Nucleotide

• Length: 1068 bp

>T174150 NZ_CP080556:c2636848-2636582 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20449.41 Da
• Isoelectric Point: 7.8569

>AT174150 WP_134826574.1 NZ_CP062251:c2749704-2749147 [Pseudomonas sp. IzPS59]
MPDTLLKPSLPGIALRRWRVLHRVKQSHAAELFKVTQSTISRWESGVQAMDPDAHRQLETLLAARLDSAADQALARLVTE
SARPVHLVCDLTHRLLACSRARAAQFSSPLSDLLGQSLWRFATAEIQHKESLLDDTGWREVQAPAALEFLTGSNDSALVP
IQPSLCRWTRIALSDGTAARLVETL

Nucleotide

• Length: 558 bp

>AT174150 NZ_CP080556:c2637105-2636848 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA