Detailed information of TA system TA174148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1803370..1804409
• Replicon: chromosome
• Accession: NZ_CP062251
• Organism: Pseudomonas sp. IzPS59

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IF189_RS08190
• Protein ID: WP_192553104.1
• Coordinates: 1803370..1803945 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: J2X4E7
• Locus tag: IF189_RS08195
• Protein ID: WP_007959254.1
• Coordinates: 1803942..1804409 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF189_RS08165	1798700..1800688	+	1989	WP_192553102.1	heme lyase CcmF/NrfE family subunit	-
IF189_RS08170	1800685..1801221	+	537	WP_011333088.1	DsbE family thiol:disulfide interchange protein	-
IF189_RS08175	1801218..1801688	+	471	WP_114881915.1	cytochrome c-type biogenesis protein CcmH	-
IF189_RS08180	1801685..1802884	+	1200	WP_192553103.1	c-type cytochrome biogenesis protein CcmI	-
IF189_RS08185	1802910..1803317	+	408	WP_085710507.1	hypothetical protein	-
IF189_RS08190	1803370..1803945	-	576	WP_192553104.1	PIN domain-containing protein	Toxin
IF189_RS08195	1803942..1804409	-	468	WP_007959254.1	helix-turn-helix domain-containing protein	Antitoxin
IF189_RS08200	1804495..1805382	-	888	WP_192553105.1	LysR family transcriptional regulator	-
IF189_RS08205	1805552..1805956	+	405	WP_114881918.1	type II toxin-antitoxin system HicB family antitoxin	-
IF189_RS08210	1805994..1807181	-	1188	WP_085710513.1	MFS transporter	-
IF189_RS08215	1807425..1808045	+	621	WP_134825906.1	hypothetical protein	-
IF189_RS08220	1808101..1809099	-	999	WP_119428798.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21664.88 Da
• Isoelectric Point: 5.9178

>T174148 WP_192553104.1 NZ_CP062251:c1803945-1803370 [Pseudomonas sp. IzPS59]
VRHSSFTALYDACVLYPAPLRDFLMWLALSGRFRARWTQEIHCEWKRNLLKNRPDLTMEQLDRTSNLMDQAIPDACVHDY
EDLIAGLTLPDCDDRHVLAAAIRCGASVIVTFNQKDFPCRSLEPFGIEAQHPDEFVDNLLGLDPAAVVAAAQKQRRQLKM
PAMDVEPFLDLLQRQGLTKSVQALSSYHSIL

Nucleotide

• Length: 576 bp

>T174148 NZ_CP080556:c2580000-2579734 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17272.70 Da
• Isoelectric Point: 6.4962

>AT174148 WP_007959254.1 NZ_CP062251:c1804409-1803942 [Pseudomonas sp. IzPS59]
MSTVIHPSISLPVEREVKAAIEGQRALAAYLATHFETQHIQIFDEQNEAHRVELPTSALRLLIDILAELAAGNAVQVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKTRRTTASEQAMALLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT174148 NZ_CP080556:c2580251-2580000 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: J2X4E7