Detailed information of TA system TA174146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 350779..351418
• Replicon: chromosome
• Accession: NZ_CP062251
• Organism: Pseudomonas sp. IzPS59

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T0K2L6
• Locus tag: IF189_RS01540
• Protein ID: WP_064384144.1
• Coordinates: 350779..350961 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IF189_RS01545
• Protein ID: WP_041475113.1
• Coordinates: 350993..351418 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF189_RS01505	346411..346788	+	378	WP_007952618.1	GNAT family N-acetyltransferase	-
IF189_RS01510	346785..347501	+	717	WP_085608636.1	AzlC family ABC transporter permease	-
IF189_RS01515	347480..347797	+	318	WP_007952614.1	AzlD domain-containing protein	-
IF189_RS01520	347714..348640	-	927	WP_085712740.1	LysR family transcriptional regulator	-
IF189_RS01525	349020..349238	+	219	WP_065257763.1	4-oxalocrotonate tautomerase family protein	-
IF189_RS01530	349288..349998	+	711	WP_192552718.1	SDR family oxidoreductase	-
IF189_RS01535	350056..350322	-	267	WP_192552719.1	DUF3077 domain-containing protein	-
IF189_RS01540	350779..350961	+	183	WP_064384144.1	type II toxin-antitoxin system HicA family toxin	Toxin
IF189_RS01545	350993..351418	+	426	WP_041475113.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IF189_RS01550	351484..351999	+	516	WP_192552720.1	hypothetical protein	-
IF189_RS01555	352477..353556	+	1080	WP_192552721.1	hypothetical protein	-
IF189_RS01560	353995..354312	+	318	WP_192553551.1	transcriptional regulator	-
IF189_RS01565	354302..354586	+	285	WP_007952602.1	helix-turn-helix domain-containing protein	-
IF189_RS01575	354868..355704	-	837	WP_192552722.1	FkbM family methyltransferase	-
IF189_RS01580	355783..356052	+	270	WP_192552723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6810.86 Da
• Isoelectric Point: 10.8173

>T174146 WP_064384144.1 NZ_CP062251:350779-350961 [Pseudomonas sp. IzPS59]
MKYSEFRRWLKAQGVEFQTGKGSHFKVSLNGRSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T174146 NZ_CP080556:c2256435-2256226 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15486.55 Da
• Isoelectric Point: 4.3686

>AT174146 WP_041475113.1 NZ_CP062251:350993-351418 [Pseudomonas sp. IzPS59]
MFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPALTAAK
VGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Nucleotide

• Length: 426 bp

>AT174146 NZ_CP080556:c2256740-2256447 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A3T0K2L6