Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3034974..3035628 Replicon chromosome
Accession NZ_CP062249
Organism Escherichia coli strain AML002_ev01

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IHN46_RS14740 Protein ID WP_000244777.1
Coordinates 3034974..3035381 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IHN46_RS14745 Protein ID WP_000354046.1
Coordinates 3035362..3035628 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN46_RS14720 3030931..3032664 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
IHN46_RS14725 3032670..3033380 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IHN46_RS14730 3033405..3034301 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IHN46_RS14735 3034413..3034934 + 522 WP_001055874.1 flavodoxin FldB -
IHN46_RS14740 3034974..3035381 - 408 WP_000244777.1 toxin CptA Toxin
IHN46_RS14745 3035362..3035628 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IHN46_RS14750 3035871..3036851 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IHN46_RS14755 3037047..3037706 - 660 WP_000250274.1 hemolysin III family protein -
IHN46_RS14760 3037870..3038181 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IHN46_RS14765 3038226..3039659 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IHN46_RS14770 3039716..3040459 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T174090 WP_000244777.1 NZ_CP062249:c3035381-3034974 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T174090 NZ_CP080536:c2113975-2113601 [Acidithiobacillus ferriphilus]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGGGACACACATCTCTCTACCCTGCTCGATCATGG
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCCTGCGGCAACCTCAAAAATCGGCAACAGACCTTATATCTGC
TCTCATGCTTACCCCGATGCACCGCCACCTCTCCAGAGGAAACCCTGTTTTTCATTGAGCGTCATGACCTGATGGGACGC
GGTATAGGTTATGTTGACGCGAGTATTCTGGCCTCTGCCGTGCTCGTGCAGGCCAATTTATGGTCCCGCGACAAACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGTGCTTACTCGGCGGAGTCATCTGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT174090 WP_000354046.1 NZ_CP062249:c3035628-3035362 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT174090 NZ_CP080536:c2114169-2113972 [Acidithiobacillus ferriphilus]
ATGCGAACTACGATCAACCTGGATGATGATCTCCTGGCCCAAGCCAAAACTCTCTGTGGCGTAGAAGACCGTGGCGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCTCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAGCATTCCTCGCAGACGAGAGCCTGCTGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References