Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2905314..2905897 Replicon chromosome
Accession NZ_CP062249
Organism Escherichia coli strain AML002_ev01

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IHN46_RS14175 Protein ID WP_000254738.1
Coordinates 2905314..2905649 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IHN46_RS14180 Protein ID WP_000581937.1
Coordinates 2905649..2905897 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN46_RS14160 2901201..2902499 - 1299 WP_000036723.1 phosphopyruvate hydratase -
IHN46_RS14165 2902587..2904224 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IHN46_RS14170 2904452..2905243 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IHN46_RS14175 2905314..2905649 - 336 WP_000254738.1 endoribonuclease MazF Toxin
IHN46_RS14180 2905649..2905897 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IHN46_RS14185 2905975..2908209 - 2235 WP_000226815.1 GTP pyrophosphokinase -
IHN46_RS14190 2908257..2909558 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T174089 WP_000254738.1 NZ_CP062249:c2905649-2905314 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T174089 NZ_CP080536:c2051919-2051536 [Acidithiobacillus ferriphilus]
ATGTGTATTTACTTGATAAAGCATCAGCCTGAGAAAGTCGCTCAACGTTTCGCTCAGTGCTATGTAGGTGACGTAGTGAT
GTCGGCTATTACCTACGCGGAACTGGAATATGGCGTTGCCGTTTCCGCAGATCCTGATCGCGAACGGGCTAACCTTGCGG
CGCTGACAGAAGACATCCCGGTGATCGCTTTCGATGCACCGGCTGGCGTTGCCTATGGCCCGATCCGGCAGGCAACCAGC
GAAAGCAAGAGAGATACGCTGGATAAGCTCATTGCCGCCCATGCCGTCGCGCTAGGCGTGACGATCGTTACCAACAAAAC
AAGGGATTTTGCCAAATATCCGGGCGTCATGATCGAGAACTGGTTGTCATCCGGTGCGCCATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT174089 WP_000581937.1 NZ_CP062249:c2905897-2905649 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT174089 NZ_CP080536:c2052182-2051946 [Acidithiobacillus ferriphilus]
ATGCACACCACTCGCGTTTTCAAAAACGGCAATTCGCAAGCAGTGCGAATCCCTGCCGATCTAGCCTATGAGCGCTCCGA
CATAGAAGTGGAAATCGAGCGGGAAGGCGACGAAATCCGCATTCGCCCCGCCCACCGCCCGCTAGCCGGCGTGCTGTCGA
AGTTTGCCCGGTTCTCTCCCAACTTTATGGCGGATGGTCGTGGCGACCAGGAACAAACCGAGAGAGACACGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References