Detailed information of TA system TA174087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2760476..2761913
• Replicon: chromosome
• Accession: NZ_CP062249
• Organism: Escherichia coli strain AML002_ev01

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: IHN46_RS13440
• Protein ID: WP_000155570.1
• Coordinates: 2760476..2761549 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: IHN46_RS13445
• Protein ID: WP_000461704.1
• Coordinates: 2761542..2761913 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHN46_RS13425	2755909..2758098	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
IHN46_RS13430	2758095..2759711	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
IHN46_RS13435	2760071..2760334	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
IHN46_RS13440	2760476..2761549	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
IHN46_RS13445	2761542..2761913	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
IHN46_RS13450	2762268..2763131	+	864	WP_000787597.1	GTPase family protein	-
IHN46_RS13455	2763223..2764044	+	822	WP_000197391.1	DUF945 domain-containing protein	-
IHN46_RS13460	2764261..2764962	+	702	WP_000189409.1	WYL domain-containing protein	-
IHN46_RS13465	2765003..2765239	+	237	WP_001144029.1	hypothetical protein	-
IHN46_RS13470	2765239..2765682	+	444	WP_000824222.1	hypothetical protein	-
IHN46_RS13475	2765706..2766173	+	468	WP_000702495.1	hypothetical protein	-
IHN46_RS13480	2766398..2766712	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T174087 WP_000155570.1 NZ_CP062249:2760476-2761549 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T174087 NZ_CP080536:c928188-927934 [Acidithiobacillus ferriphilus]
GTGAGGCTGATCTTCTCTGACGATGCATGGGAAGACTACCTATATTGGCAGAAGCAAGACAAGCGCGTGGTCGAGCGAAT
CAATAAGCTGATACGTGAGACACAGCGTGAACCCTTCGCGGGAGTTGGCAAACCCGAAGCACTGAAGCATGCTCTTTCAG
GATTTTGGTCACGCCGTATTACAGATGAGCATCGCATGGTGTATCGAATAGAAGGTGATGCATTGATGATTGCTCAGTTG
CGTTTCCACTACTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT174087 WP_000461704.1 NZ_CP062249:2761542-2761913 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT174087 NZ_CP080536:c928436-928185 [Acidithiobacillus ferriphilus]
ATGGATGCGATTACCTATACCACCGTTCGTGCAAATCTCGCCAGCGCCATGGACCGCGTTTGCGACGACCACGAACCCTT
GATCATTACCCGCAATGGCGAACAGTCCGTCGTGATGCTCTCGCTCGAAGACTACAAGGCGCTGGAGGAAACCGCCTACC
TCTTGCGTACCCCCGCCAACGCCAAGCGCCTTCTCGCTGCCGTCGCACAACTGAACGCCGGTAAAGGCGTTGAGCGGAAG
CTGGCGAAGTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4