Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2071792..2072623 Replicon chromosome
Accession NZ_CP062249
Organism Escherichia coli strain AML002_ev01

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IHN46_RS10245 Protein ID WP_000854814.1
Coordinates 2072249..2072623 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag IHN46_RS10240 Protein ID WP_001285584.1
Coordinates 2071792..2072160 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN46_RS10225 2069459..2070991 + 1533 WP_001350525.1 hypothetical protein -
IHN46_RS10230 2070988..2071434 + 447 WP_000187523.1 RadC family protein -
IHN46_RS10235 2071497..2071718 + 222 WP_000692323.1 DUF987 domain-containing protein -
IHN46_RS10240 2071792..2072160 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
IHN46_RS10245 2072249..2072623 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
IHN46_RS10250 2072620..2072814 + 195 WP_000988600.1 hypothetical protein -
IHN46_RS10255 2072827..2072940 + 114 WP_001161660.1 DUF957 domain-containing protein -
IHN46_RS10260 2073229..2073357 - 129 Protein_2004 transposase domain-containing protein -
IHN46_RS10265 2073429..2073611 + 183 WP_001016348.1 hypothetical protein -
IHN46_RS10270 2073712..2074041 - 330 WP_000450409.1 DUF496 family protein -
IHN46_RS10275 2074213..2075271 - 1059 WP_001200891.1 FUSC family protein -
IHN46_RS10280 2075469..2075942 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
IHN46_RS10285 2076061..2077227 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T174085 WP_000854814.1 NZ_CP062249:2072249-2072623 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T174085 NZ_CP080536:865752-866078 [Acidithiobacillus ferriphilus]
ATGATGCGCGGCGATCTTGTGACCATCGCCATGCAGGGCGACTTTGGCAAGCCACGGCCCGCGTTGGTGATTCAAGCCAA
CCTGTTTGGGGAGCACACGAGCGTAACCGTTCTGCCAATCACCAGCACGCTGGTTGCTGCGCCATTGCTGCGCGTCACGG
TTCAACCGAGCGCAGAAAACGGCTTGCAGAAGCCATCACAGGTGATGGTGGACAAGGCGATGACGGTGAAGCGCGAAAAG
GCAGGGCCAGTATTCGGACGCATCGACGCGGATGCACTGGTGGAGGTCGAGCGTTGCTTGGCTGTGTTTTTGGGGATAGC
GAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT174085 WP_001285584.1 NZ_CP062249:2071792-2072160 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT174085 NZ_CP080536:865531-865755 [Acidithiobacillus ferriphilus]
ATGGGAACCACGCACGTTAATACACGAGTTCAAAAGCACCGCGACGCGCTGCGCATGGCGGGCTTGCGCCCGGTGCAAAT
CTGGGTGCCTGACACACGCCGCCCAGACTTCGCGGAGGAATGTCGCCGCCAATCCCTTCTCGTGGCTCAAGCCGACAGCG
CCGATACAGCCATGCTGCAGTTCATGGATGAAGTCTTGGCAGATGTGGACGGTTGGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References