Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4343604..4344101 Replicon chromosome
Accession NZ_CP062247
Organism Escherichia coli strain AML002_ev03

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IHN48_RS20910 Protein ID WP_001173343.1
Coordinates 4343928..4344101 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IHN48_RS20905 Protein ID WP_000398619.1
Coordinates 4343604..4343900 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN48_RS20885 4340335..4341054 - 720 WP_000611288.1 two-component system response regulator DcuR -
IHN48_RS20890 4341051..4342682 - 1632 WP_001216477.1 sensor histidine kinase -
IHN48_RS20895 4342863..4343093 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
IHN48_RS20900 4343105..4343377 + 273 WP_000405651.1 N-acetyltransferase -
IHN48_RS20905 4343604..4343900 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IHN48_RS20910 4343928..4344101 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IHN48_RS20915 4344220..4345737 - 1518 WP_001295074.1 lysine--tRNA ligase -
IHN48_RS20920 4345974..4347431 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T174010 WP_001173343.1 NZ_CP062247:4343928-4344101 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T174010 NZ_CP080478:c1407718-1407440 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTACTGGGTCGAGGGCGATATCATCAGAGCTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT174010 WP_000398619.1 NZ_CP062247:4343604-4343900 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT174010 NZ_CP080478:c1407999-1407715 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCAGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References