Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2905318..2905901 Replicon chromosome
Accession NZ_CP062247
Organism Escherichia coli strain AML002_ev03

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IHN48_RS14175 Protein ID WP_000254738.1
Coordinates 2905318..2905653 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IHN48_RS14180 Protein ID WP_000581937.1
Coordinates 2905653..2905901 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN48_RS14160 2901205..2902503 - 1299 WP_000036723.1 phosphopyruvate hydratase -
IHN48_RS14165 2902591..2904228 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IHN48_RS14170 2904456..2905247 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IHN48_RS14175 2905318..2905653 - 336 WP_000254738.1 endoribonuclease MazF Toxin
IHN48_RS14180 2905653..2905901 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IHN48_RS14185 2905979..2908213 - 2235 WP_000226815.1 GTP pyrophosphokinase -
IHN48_RS14190 2908261..2909562 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T174003 WP_000254738.1 NZ_CP062247:c2905653-2905318 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T174003 NZ_CP080470:c3742307-3741897 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCGA
CGCAGGAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT174003 WP_000581937.1 NZ_CP062247:c2905901-2905653 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT174003 NZ_CP080470:c3742554-3742288 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References