Detailed information of TA system TA173988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1504746..1505384
• Replicon: chromosome
• Accession: NZ_CP062247
• Organism: Escherichia coli strain AML002_ev03

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IHN48_RS07365
• Protein ID: WP_000813794.1
• Coordinates: 1504746..1504922 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IHN48_RS07370
• Protein ID: WP_001270286.1
• Coordinates: 1504968..1505384 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHN48_RS07345	1500365..1501579	-	1215	WP_001326689.1	BenE family transporter YdcO	-
IHN48_RS07350	1501632..1502168	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IHN48_RS07355	1502241..1504202	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IHN48_RS07360	1504294..1504524	-	231	WP_000494244.1	YncJ family protein	-
IHN48_RS07365	1504746..1504922	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IHN48_RS07370	1504968..1505384	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IHN48_RS07375	1505463..1506869	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
IHN48_RS07380	1507114..1508259	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IHN48_RS07385	1508277..1509290	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
IHN48_RS07390	1509291..1510232	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T173988 WP_000813794.1 NZ_CP062247:1504746-1504922 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T173988 NZ_CP080466:978248-978565 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT173988 WP_001270286.1 NZ_CP062247:1504968-1505384 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT173988 NZ_CP080466:977987-978229 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Structures

Toxin

• PDB: 6HPB