Detailed information of TA system TA173958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2759277..2760714
• Replicon: chromosome
• Accession: NZ_CP062246
• Organism: Escherichia coli strain AML003_par

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: IHN49_RS13430
• Protein ID: WP_000155570.1
• Coordinates: 2759277..2760350 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: IHN49_RS13435
• Protein ID: WP_000461704.1
• Coordinates: 2760343..2760714 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHN49_RS13415	2754710..2756899	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
IHN49_RS13420	2756896..2758512	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
IHN49_RS13425	2758872..2759135	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
IHN49_RS13430	2759277..2760350	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
IHN49_RS13435	2760343..2760714	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
IHN49_RS13440	2761069..2761932	+	864	WP_000787597.1	GTPase family protein	-
IHN49_RS13445	2762024..2762845	+	822	WP_000197391.1	DUF945 domain-containing protein	-
IHN49_RS13450	2763062..2763763	+	702	WP_000189409.1	WYL domain-containing protein	-
IHN49_RS13455	2763804..2764040	+	237	WP_001144029.1	hypothetical protein	-
IHN49_RS13460	2764040..2764483	+	444	WP_000824222.1	hypothetical protein	-
IHN49_RS13465	2764507..2764974	+	468	WP_000702495.1	hypothetical protein	-
IHN49_RS13470	2765199..2765513	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T173958 WP_000155570.1 NZ_CP062246:2759277-2760350 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T173958 NZ_CP080458:1217981-1218229 [Staphylococcus carnosus]
ATGGCTGTTGAAACATACTCAAATGCTAGAAAAAACTTTAGAAGTTTAATTAAACAAGTAAATGAAAACAGTGAAGCTAT
TACCATCACAACAAATGAAGAAAACGCCGTTTTAATGTCTGAAACTGATTATAATGCAATAATGGAGACTCTTTACTTAC
AACAAAACCCAGCTAACGCTGCACATCTCAAACGTTCAATTGAACAACTAGAAAAAGGCGATTCTGTTCAGGTAGAAATA
GATGAGTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT173958 WP_000461704.1 NZ_CP062246:2760343-2760714 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT173958 NZ_CP080458:1218222-1218491 [Staphylococcus carnosus]
ATGAGTAAATTAAATATTTCATTTTCTCCTGAAGCATTTGAAGATTATAAAAATTGGCAAACTCAAGATAAAAAAGTTTG
GAAGAAAATCAATCAATTGATTAAAAGTATTGATCGTGATGGCATGTTAGATGGAATTGGTAAGCCAGAACGTTTAAAAG
GTGGTTTCAGTGGTTGGTATAGCAGACGTATCACACAAGATCACAGATTAGTATATAAAGTAACAAGTGAATCAATTTTA
GCTGCTTCATGCAAGTATCATTATCAATAA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4