Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2070592..2071423 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IHN50_RS10235 Protein ID WP_000854814.1
Coordinates 2071049..2071423 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag IHN50_RS10230 Protein ID WP_001285584.1
Coordinates 2070592..2070960 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS10215 2068259..2069791 + 1533 WP_001350525.1 hypothetical protein -
IHN50_RS10220 2069788..2070234 + 447 WP_000187523.1 RadC family protein -
IHN50_RS10225 2070297..2070518 + 222 WP_000692323.1 DUF987 domain-containing protein -
IHN50_RS10230 2070592..2070960 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
IHN50_RS10235 2071049..2071423 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
IHN50_RS10240 2071420..2071614 + 195 WP_000988600.1 hypothetical protein -
IHN50_RS10245 2071627..2071740 + 114 WP_001161660.1 DUF957 domain-containing protein -
IHN50_RS10250 2072029..2072157 - 129 Protein_2002 transposase domain-containing protein -
IHN50_RS10255 2072229..2072411 + 183 WP_001016348.1 hypothetical protein -
IHN50_RS10260 2072512..2072841 - 330 WP_000450409.1 DUF496 family protein -
IHN50_RS10265 2073013..2074071 - 1059 WP_001200891.1 FUSC family protein -
IHN50_RS10270 2074269..2074742 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
IHN50_RS10275 2074861..2076027 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T173913 WP_000854814.1 NZ_CP062245:2071049-2071423 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T173913 NZ_CP080380:1769394-1769675 [Microbacterium sp. PAMC22086]
GTGATCAGATCGTTCGGCAACAAGGACACCGAACGGCTGTGGCGTCGTGAACGCGTGCGCTCGATCGATCCGAGGATCCA
CCGAGTCGCGTTGCGGAAATTGCGCCAGGTGGGCTCAGCGGAGGTGCTCGATGACCTCCGCATCCCACCAGGCAACAGGC
TCGAAGCGCTGAGAGGCGATCGAGCCGGGCAGCACAGCATCAGAATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACG
GACGCCGGACCGGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT173913 WP_001285584.1 NZ_CP062245:2070592-2070960 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT173913 NZ_CP080380:1769662-1769964 [Microbacterium sp. PAMC22086]
TTGACTACCACTGACAGGATCGAGCCGATCCACCCAGGTGAGGTCCTCATGGAGGACTTCATCGAGGGCTTCGGCATCAC
ACAGAACAAGCTCGCCGTGTCGATCGGCGTCCCGCCCCGGCGGATCAACGAGATCGTGCACGGCAAGCGGGGGATCACCG
CAGACACGGCGCTGCGGCTCGCGAGGTACTTCGGCACGTCGGCCGAGTTCTGGATCAACCTGCAGAGCCACTATGAGCTC
GACCGCGCCGAGGACGCAGCAGGTGAGCAGATCGCGGCGATCGCGCCGCTACAGGTCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References