Detailed information of TA system TA173666

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4342401..4342898
• Replicon: chromosome
• Accession: NZ_CP062239
• Organism: Escherichia coli strain AML001_ev03

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: IHN56_RS20900
• Protein ID: WP_001173343.1
• Coordinates: 4342725..4342898 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: IHN56_RS20895
• Protein ID: WP_000398619.1
• Coordinates: 4342401..4342697 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHN56_RS20875	4339132..4339851	-	720	WP_000611288.1	two-component system response regulator DcuR	-
IHN56_RS20880	4339848..4341479	-	1632	WP_001216477.1	sensor histidine kinase	-
IHN56_RS20885	4341660..4341890	+	231	WP_000371704.1	(4Fe-4S)-binding protein	-
IHN56_RS20890	4341902..4342174	+	273	WP_000405651.1	N-acetyltransferase	-
IHN56_RS20895	4342401..4342697	+	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
IHN56_RS20900	4342725..4342898	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
IHN56_RS20905	4343017..4344534	-	1518	WP_001295074.1	lysine--tRNA ligase	-
IHN56_RS20910	4344771..4346228	-	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T173666 WP_001173343.1 NZ_CP062239:4342725-4342898 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T173666 NZ_CP080260:957422-958369 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTACTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACAGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TGGTGCAGGCACCTTGTTTACGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCCTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCTGAAAAGCTGGTGGCGATGTTACGCCGTACAGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAACGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGCTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGTTATCCATCCTGAATGCTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT173666 WP_000398619.1 NZ_CP062239:4342401-4342697 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT173666 NZ_CP080260:957034-957429 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGATCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGTAGATTAAATGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: A0A7U9QBH6