Detailed information of TA system TA173629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 130988..132471
• Replicon: plasmid pTH2a
• Accession: NZ_CP062232
• Organism: Rhizobium sp. TH2

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: IHQ71_RS29485
• Protein ID: WP_258163036.1
• Coordinates: 131212..132471 (+)
• Length: 420 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: IHQ71_RS29480
• Protein ID: WP_258163235.1
• Coordinates: 130988..131209 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHQ71_RS29465 (IHQ71_29355)	126951..127802	+	852	WP_258163032.1	FAD binding domain-containing protein	-
IHQ71_RS29470 (IHQ71_29360)	127802..128272	+	471	WP_258163034.1	(2Fe-2S)-binding protein	-
IHQ71_RS29475 (IHQ71_29365)	128269..130626	+	2358	WP_258163035.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IHQ71_RS29480 (IHQ71_29370)	130988..131209	+	222	WP_258163235.1	type II toxin-antitoxin system Y4mF family antitoxin	Antitoxin
IHQ71_RS29485 (IHQ71_29375)	131212..132471	+	1260	WP_258163036.1	type II toxin-antitoxin system HipA family toxin	Toxin
IHQ71_RS29490 (IHQ71_29380)	132481..134808	-	2328	Protein_115	hypothetical protein	-
IHQ71_RS29495 (IHQ71_29385)	134958..135835	+	878	Protein_116	HNH endonuclease	-
IHQ71_RS29500 (IHQ71_29390)	136248..136487	+	240	WP_258163037.1	hypothetical protein	-
IHQ71_RS29505 (IHQ71_29395)	136837..136970	+	134	Protein_118	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..238315	238315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-352)

Antitoxin

• Transcription regulator, HipB-like (9-65)

Sequences

Toxin

Protein

• Length: 420 a.a.
• Molecular weight: 45573.03 Da
• Isoelectric Point: 5.8782

>T173629 WP_258163036.1 NZ_CP062232:131212-132471 [Rhizobium sp. TH2]
VTRYLDVYFSNIKAGTLGQDADGTLTYSYDVEYLRDGGVAAISFSMPLREEPYLDQIVRPFFSGLLPDEGARQRLAGALG
LSSGNAFGLLEVIGGECAGALSLHRAGEAPALNDDEGVEELSGERLSDVIGKLRSRPLLGGEEGVRLSLAGAQDKLAVIA
QDDTIGLAKGGRPTTHILKPVIQGLEGTVENELFCLRLADRLKLPVPTAEMRWSNETAFLLIERYDRARASHGRIDRLHQ
EDFCQALSVPPELKYEEEGGPSTERSVDLINRACGRPAADRLRFIRMLIFHYLVGNADAHGKNYALLYDGKSPDLAPLYD
VVCTAAYPRLAKKLAMKIGGRAVPDTTQLKHWLTLVPDTKAAQRLLVSDIANLAGNIESEADALLLEFSDAGVKHPVLKA
VRGIVGTRAAHLQRIIERV

Nucleotide

• Length: 1260 bp

>T173629 NZ_CP080253:c2841964-2841581 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7931.10 Da
• Isoelectric Point: 9.4941

>AT173629 WP_258163235.1 NZ_CP062232:130988-131209 [Rhizobium sp. TH2]
MDTVSDTNTLGMLIRAERKSQSLTQEQLAGIAGVGVRFVRELEAGKESCQLGRALQVAQTLGLVLSVSTRRER

Nucleotide

• Length: 222 bp

>AT173629 NZ_CP080253:c2842425-2842045 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG