Detailed information of TA system TA173577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3278903..3279942
• Replicon: chromosome
• Accession: NZ_CP062218
• Organism: Pseudomonas putida strain SY153

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IGB31_RS15120
• Protein ID: WP_100773622.1
• Coordinates: 3279367..3279942 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A7L5F0B4
• Locus tag: IGB31_RS15115
• Protein ID: WP_043156233.1
• Coordinates: 3278903..3279370 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGB31_RS15080	3274276..3275694	-	1419	WP_100773628.1	TIGR03752 family integrating conjugative element protein	-
IGB31_RS15085	3275684..3276610	-	927	WP_100773627.1	TIGR03749 family integrating conjugative element protein	-
IGB31_RS15090	3276607..3277296	-	690	WP_100773626.1	TIGR03746 family integrating conjugative element protein	-
IGB31_RS15095	3277293..3277691	-	399	WP_100773625.1	TIGR03750 family conjugal transfer protein	-
IGB31_RS15100	3277703..3278062	-	360	WP_100773624.1	TIGR03745 family integrating conjugative element membrane protein	-
IGB31_RS15105	3278079..3278312	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
IGB31_RS15110	3278309..3278689	-	381	WP_100773623.1	RAQPRD family integrative conjugative element protein	-
IGB31_RS15115	3278903..3279370	+	468	WP_043156233.1	helix-turn-helix domain-containing protein	Antitoxin
IGB31_RS15120	3279367..3279942	+	576	WP_100773622.1	PIN domain-containing protein	Toxin
IGB31_RS15125	3279960..3280874	+	915	WP_100773621.1	AAA family ATPase	-
IGB31_RS15130	3280871..3281341	+	471	WP_100773620.1	hypothetical protein	-
IGB31_RS15135	3281338..3281838	+	501	WP_100773619.1	HORMA domain containing protein	-
IGB31_RS15140	3281838..3282740	+	903	WP_100773618.1	nucleotidyltransferase domain-containing protein	-
IGB31_RS15145	3282777..3283502	+	726	WP_100773617.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3226057..3323360	97303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21570.58 Da
• Isoelectric Point: 5.6513

>T173577 WP_100773622.1 NZ_CP062218:3279367-3279942 [Pseudomonas putida]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQTIHEEWKRNLLINRPDLTRAQVDRTSDLMDRAIPDGLVEGY
ESLAAGLTLPDPDDRHVLAAAIRCGASVIVTFNQRDFPNELLAPYGVESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLRN
PTIDVDRYLDILLRQGLVQTTKVLAGYRTIL

Nucleotide

• Length: 576 bp

>T173577 NZ_CP080247:1953979-1954082 [Escherichia sp. TM-G17TGC]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17251.62 Da
• Isoelectric Point: 5.9214

>AT173577 WP_043156233.1 NZ_CP062218:3278903-3279370 [Pseudomonas putida]
MTVTAQSKMTLPAEGEVKAAVQGQRALAAYLATQFETQHIQIFDDQKQAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELTQQSQELGMGYE

Nucleotide

• Length: 468 bp

>AT173577 NZ_CP080247:c1954120-1953849 [Escherichia sp. TM-G17TGC]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A7L5F0B4