Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 154673..155316 Replicon plasmid unnamed1
Accession NZ_CP062212
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag IFO96_RS24770 Protein ID WP_001034044.1
Coordinates 154900..155316 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag IFO96_RS24765 Protein ID WP_001261286.1
Coordinates 154673..154903 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS24750 149810..150040 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IFO96_RS24755 150037..150453 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
IFO96_RS24760 150498..154292 - 3795 WP_001144731.1 hypothetical protein -
IFO96_RS24765 154673..154903 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IFO96_RS24770 154900..155316 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
IFO96_RS24775 155391..156956 + 1566 WP_001128474.1 AAA family ATPase -
IFO96_RS24780 156941..157963 + 1023 WP_000361402.1 helicase UvrD -
IFO96_RS24785 158217..158914 - 698 Protein_161 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..177038 177038
- flank IS/Tn - - 158217..158720 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T173566 WP_001034044.1 NZ_CP062212:154900-155316 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T173566 NZ_CP080247:183644-183751 [Escherichia sp. TM-G17TGC]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT173566 WP_001261286.1 NZ_CP062212:154673-154903 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT173566 NZ_CP080247:c183595-183529 [Escherichia sp. TM-G17TGC]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References