Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4434440..4435071 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag IFO96_RS21660 Protein ID WP_001259388.1
Coordinates 4434796..4435071 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IFO96_RS21655 Protein ID WP_000593555.1
Coordinates 4434440..4434799 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS21630 4430572..4431516 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
IFO96_RS21635 4431513..4432346 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IFO96_RS21640 4432346..4433110 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IFO96_RS21645 4433107..4433913 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IFO96_RS21650 4433919..4434320 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IFO96_RS21655 4434440..4434799 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IFO96_RS21660 4434796..4435071 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
IFO96_RS21665 4435144..4436268 - 1125 WP_001314210.1 ABC transporter permease -
IFO96_RS21670 4436268..4439003 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
IFO96_RS21675 4439000..4440067 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T173554 WP_001259388.1 NZ_CP062211:c4435071-4434796 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T173554 NZ_CP080246:2861786-2862166 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT173554 WP_000593555.1 NZ_CP062211:c4434799-4434440 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT173554 NZ_CP080246:2861365-2861739 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References