Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3833485..3834139 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IFO96_RS18650 Protein ID WP_000244772.1
Coordinates 3833485..3833892 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IFO96_RS18655 Protein ID WP_000354046.1
Coordinates 3833873..3834139 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS18630 3829442..3831175 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
IFO96_RS18635 3831181..3831891 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IFO96_RS18640 3831916..3832812 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IFO96_RS18645 3832924..3833445 + 522 WP_001055874.1 flavodoxin FldB -
IFO96_RS18650 3833485..3833892 - 408 WP_000244772.1 protein YgfX Toxin
IFO96_RS18655 3833873..3834139 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IFO96_RS18660 3834382..3835362 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IFO96_RS18665 3835439..3836098 - 660 WP_000250274.1 hemolysin III family protein -
IFO96_RS18670 3836262..3836573 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IFO96_RS18675 3836618..3838051 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
IFO96_RS18680 3838108..3838851 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T173552 WP_000244772.1 NZ_CP062211:c3833892-3833485 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T173552 NZ_CP080246:c1924872-1924498 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT173552 WP_000354046.1 NZ_CP062211:c3834139-3833873 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT173552 NZ_CP080246:c1925329-1924961 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References