Detailed information of TA system TA173550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3377498..3378123
• Replicon: chromosome
• Accession: NZ_CP062211
• Organism: Escherichia coli strain L3Cip3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IFO96_RS16410
• Protein ID: WP_000911330.1
• Coordinates: 3377498..3377896 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IFO96_RS16415
• Protein ID: WP_000450524.1
• Coordinates: 3377896..3378123 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFO96_RS16390	3373405..3373605	+	201	WP_000383836.1	YpfN family protein	-
IFO96_RS16395	3373686..3374384	-	699	WP_000679812.1	esterase	-
IFO96_RS16400	3374458..3376473	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
IFO96_RS16405	3376488..3377351	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
IFO96_RS16410	3377498..3377896	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IFO96_RS16415	3377896..3378123	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IFO96_RS16420	3378277..3378990	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IFO96_RS16425	3379203..3380237	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
IFO96_RS16430	3380254..3381132	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IFO96_RS16435	3381278..3381850	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IFO96_RS16440	3381850..3382320	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T173550 WP_000911330.1 NZ_CP062211:c3377896-3377498 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T173550 NZ_CP080246:1912282-1912533 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT173550 WP_000450524.1 NZ_CP062211:c3378123-3377896 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT173550 NZ_CP080246:1912530-1912784 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33