173535

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1825879..1827599 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag IFO96_RS08755 Protein ID WP_000312833.1
Coordinates 1826943..1827599 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag IFO96_RS08750 Protein ID WP_000634203.1
Coordinates 1825879..1826946 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS08735 1821090..1821704 - 615 Protein_1699 IS3-like element IS600 family transposase -
IFO96_RS08740 1822049..1822765 - 717 WP_001122107.1 M48 family metallopeptidase -
IFO96_RS08745 1822765..1825860 - 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
IFO96_RS08750 1825879..1826946 - 1068 WP_000634203.1 macro domain-containing protein Antitoxin
IFO96_RS08755 1826943..1827599 - 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
IFO96_RS08760 1827612..1828955 - 1344 WP_032180130.1 restriction endonuclease subunit S -
IFO96_RS08765 1828967..1831405 - 2439 WP_032180025.1 type I restriction-modification system subunit M -
IFO96_RS08770 1831445..1831684 - 240 WP_024224214.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1817474..1857366 39892


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T173535 WP_000312833.1 NZ_CP062211:c1827599-1826943 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T173535 NZ_CP080238:c252671-252381 [Vibrio sp. ED002]
ATGATCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCTACTCTGCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCACATGTTCCGGCTTCAAAACTTTCTGGAGCTGACAACATGTATAAAATTA
AGTTGCGTCAATCGGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCCGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGTAAAGCCATGAAAAGGTTAGATGATTGA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT173535 WP_000634203.1 NZ_CP062211:c1826946-1825879 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT173535 NZ_CP080238:c252909-252661 [Vibrio sp. ED002]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACCGAGTTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTACGGAGAACCTGTTGCTGTATTAAACCGAAATGAGCCAGCATTTTATTGCGTACCTGCCGAAGCGTACGAAATGATGA
TGGATAGACTCGAAGATCTTGAACTACTAGCTATCGCTAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GATCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References