Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 958966..959803 Replicon chromosome
Accession NZ_CP062211
Organism Escherichia coli strain L3Cip3

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IFO96_RS04670 Protein ID WP_000227784.1
Coordinates 958966..959508 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IFO96_RS04675 Protein ID WP_001297137.1
Coordinates 959492..959803 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFO96_RS04650 954505..955416 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
IFO96_RS04655 955584..956075 + 492 WP_001138904.1 nucleotide binding protein YajQ -
IFO96_RS04660 956203..957567 - 1365 WP_001000974.1 MFS transporter -
IFO96_RS04665 957975..958910 + 936 WP_001365761.1 sel1 repeat family protein -
IFO96_RS04670 958966..959508 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IFO96_RS04675 959492..959803 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IFO96_RS04680 959988..960878 - 891 WP_000971336.1 heme o synthase -
IFO96_RS04685 960890..961219 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IFO96_RS04690 961219..961833 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IFO96_RS04695 961823..963814 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IFO96_RS04700 963836..964783 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T173533 WP_000227784.1 NZ_CP062211:c959508-958966 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T173533 NZ_CP080238:c168211-167921 [Vibrio sp. ED002]
ATGACTTATAATCTTGAATTTAAAAGTAGCGCACTTAAAGAGTGGAAAAAACTCGGCGCTACTTTGCAACAGCAATTTAA
GAAGAAACTGATTGAGCGCTTAGCTAATCCACACGTTCCGGCTTCCAAATTATCCGGCGCAGACAACATGTATAAGATCA
AATTACGCCAATCTGGCTATCGCTTAGTATACAAAGTGCAAGACGATATAGTTATTGTTACCGTTTTAGCCGTAGGCAAG
CGCGAACGTAGCGATGTGTACAGAAAAGCGATGACAAGGCTCAACGACTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT173533 WP_001297137.1 NZ_CP062211:c959803-959492 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT173533 NZ_CP080238:c168449-168201 [Vibrio sp. ED002]
ATGACCGCTAGAATATTAGCTGATGTTGCTGCAAGTATCACTGAGTTTAAAGCGAACCCAATGAAGGTTGCTTCAAGCGC
TTATGGTGATCCAGTCGCCGTACTAAACCGAAATGAACCTGCATTTTACTGTGTACCTGCTGCTGCATACGAAATGATGA
TGGACAAACTTGAAGATATTGAGCTACTTGCTTTAGCTAAAGAGCGAGAATCTGAAGCAAGCATTTCGGTGAGTATCGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References